SOLICITATION NOTICE
B -- Salmon Imprinting and Reproduction Research
- Notice Date
- 5/19/2005
- Notice Type
- Solicitation Notice
- NAICS
- 541710
— Research and Development in the Physical, Engineering, and Life Sciences
- Contracting Office
- Department of Commerce, National Oceanic and Atmospheric Administration (NOAA), Western Administrative Support Center, 7600 Sand Point Way, Northeast, Seattle, WA, 98115-6349
- ZIP Code
- 98115-6349
- Solicitation Number
- AB133F-05-RP-1169
- Response Due
- 7/5/2005
- Archive Date
- 7/20/2005
- Description
- This publication serves as a notice of intent to procure, on a sole-source basis, with the University of Washington (UW). There are three tasks to the required work: TASK 1 - Determine the effects of growth on age of sexual maturity and ovarian development in chinook salmon and determining the effects of temperature manipulation on spawn timing in spring Chinook salmon. The following are required for Task 1 work: a) rearing of Chinook and coho salmon on specified rations to control growth rate in different groups of experimental fish, b) collection of tissues and plasma from experimental fish, c) measurement of plasma and pituitary gonadotropins, plasma sex steroids, and IGF I by radioimmunossay, d) measurement of ovarian gene transcripts (gonadotopin and growth hormone receptors, IGF I and IGF II, steroid acute regulatory protein) from experimental fish by real time PCR, e) design of temperature regime to manipulate spawn time, f) computer image analysis of gonad development from histological sections of gonads from experimental fish, g) analysis of carcass samples for lipid levels, h) spawning of fish from temperature study and assessment of gamete fertility, and i) statistical analyses and interpretation of data collected from experimental fish. All analyses and fish rearing shall be conducted at the Northwest Fisheries Science Center in Seattle, WA. TASK 2 ? Assist with laboratory and behavioral assessments of olfactory imprinting by sockeye salmon. To determine the critical period(s) for imprinting by sockeye salmon, juvenile salmon are being exposed to known odorants at key developmental stages and are being tested subsequently for development of long-term memories of these ordorants. The following are require for Task 2 work: a) maintain odor exposure equipment and odorants, b) conduct routine sampling of fish for tissue and physiological assays to assess smolting and imprinting, c) assist with behavioral assessments of imprinting in experimental stream channel, d) conduct electro-olfactogram assessments of imprinted sockeye salmon, and e) assist with molecular assessments of imprinting. TASK 3 ? Assist with collecting biological information and conducting genetic and phenotypic analyses of inbreeding-study families of University of Washington stock Chinook salmon. Assistance is required to capture experimental adult fish and retrieve biological data, coded-wire tag information and genetic sampling, and assist with periodic sampling of juvenile study fish for size, growth, and maturation. Modest assistance in conducting DNA microsatellite analyses of the family data for estimation of imbreeding coefficients and for maintaining pedigree records for the study is required. All analyses and fish rearing will be conducted at the NOAA, National Marine Fisheries Service, Northwest Fisheries Science Center, Seattle, WA. The period of performance will begin on date of contract award (approx. June 2005) and continue through September 30, 2006.----The Government intends to negotiate on a sole-source basis with the UW, as we believe this is the only source with such unique expertise, qualifications and experience to perform the work. All interested concerns must submit documentation in writing demonstrating the capability, expertise, qualifications and experience to perform the necessary work to Sharon Kent, NOAA, WASC, 7600 Sand Point Way NE, Seattle WA. This notice may represent the only official notice of such solicitation. See numbered Note 22.----All contractors doing business with the Government are now required to be registered with the Central Contractor Registry (CCR). For additional information and to register in CCR please access the following web site: http://www.ccr.gov. In order to register with the CCR and to be eligible to receive an award, all offerors must have a Dunn & Bradstreet Number. A Dunn & Bradstreet number may be acquired free of charge by contacting Dunn & Bradstreet on-line at https://www.dnb.com/product/eupdate/requestOptions.html or by phone at (800) 333-0505.
- Place of Performance
- Address: Seattle, WA
- Record
- SN00810951-W 20050521/050519211809 (fbodaily.com)
- Source
-
FedBizOpps.gov Link to This Notice
(may not be valid after Archive Date)
| FSG Index | This Issue's Index | Today's FBO Daily Index Page |