SOLICITATION NOTICE
Q -- Cytogenetics testing
- Notice Date
- 9/14/2006
- Notice Type
- Solicitation Notice
- NAICS
- 541380
— Testing Laboratories
- Contracting Office
- Department of Veterans Affairs;ASC - VISN 9;3400 Lebanon Pike;Murfreesboro TN 37129
- ZIP Code
- 37129
- Solicitation Number
- VA-249-06-RP-0062
- Response Due
- 9/30/2007
- Archive Date
- 10/30/2007
- Description
- SPECIFICATIONS/SCOPE OF WORK Service for bone marrow and peripheral blood cytogenetics will be performed on an on call and emergency basis, twenty four hours a day, seven days a week, including holidays and weekends. Government holidays are: New Year's Day, Washington's Birthday, Memorial Day, Independence Day, Labor Day, Columbus Day, Veterans Day, Thanksgiving, Christmas Day and Martin Luther King's Birthday. The VAMC Laboratory Service will contact the contractor at the phone number provided to the VAMC after award. The samples will be picked up by the contractor from VAMC Bone Marrow Lab, Room C-236A, 1310 24th Avenue South, Nashville, TN 37212-2637 no later than one hour after contacted by the VAMC. On completion of procedures accomplished on an emergency basis, an oral preliminary report of the results will be provided to the VAMC within twenty-four hours. On completion of routine services, a fixed report of preliminary results will be provided to the VAMC within three (3) calendar days at 615-321-6327 followed by a written report within seven (7) calendar days. The written and oral report will be provided to the Bone Marrow Lab, Hematology Research Lab, (111C). Attn: Amanda Hedges, Bone Marrow Technician, at 327-4751 extension 5501, VAMC, Nashville, TN. Payment will not be issued until written reports are received. The head of the Laboratory must be certified in Bone Marrow Cytogenetics. The prices submitted are based on analysis of 20 G-banded metaphases and a minimum of 2 karyotypes. Additional karyotypes will be produced on every abnormal clone observed. Additional banding such a Q-,C-, and NOR will be performed, when required at no additional cost. The Laboratory must be licensed by the State of Tennessee. Personnel assigned by the contractor to perform the services covered by this contract shall be licensed in a State, Territory, or Commonwealth of the United States or the District of Columbia. The qualifications of such personnel shall also be subject to review by the VA Chief of Staff and approval by the VA Facility Director. In accordance with applicable confidentiality laws, copies of clinical or other medical records of VA beneficiaries treated by the contractor will be forwarded to VA. Protocol for chromosome analysis in leukemia and myelodysplastic syndromes: I. Samples Studied: Bone Marrow aspirates, peripheral bloods or spleen samples are provided to the cytogenetic laboratory with a requisition form describing the tentative diagnosis. In cases of ANNL, CML, and MDS chromosome preparation is done for all samples. In cases of lymphocytic leukemia a direct preparation as well as a preparation after one to three days' cultures are done. These multiple analysis are done because culture is required to detect some anomalies after an overnight culture. II. Cultures, Harvesting, and Chromosome Preparation Techniques: Collect 1-3 mls of samples in a sodium heparin tube (green top) Add 2 mls of Hanks balanced salt solution containing a 2.5 ug of colcemid/ml and 4 mls of Hank's balanced salt solution to a sterile 15 ml Corning graduated tube. Add 1.0 ml of sample to colcemid in corning tube. Mix gently. Leave at room temperature for 45 minutes. After colcemid treatment, spin tube at 1200 rpm for 5 minutes and decant supernatant. Add 1.0 ml of prewarmed 0.56 M KCI (0.56 g KCL/100 ml H20), and suspend. Plate tube in 37 C water bath for 25 minutes. Add 1.0 ml fixer (1part flacial acetic acid to 3 parts absolute methanol), and centrifuge. Remove supernatant and add 10 ml. Fresh fixer, suspend and spin. Repeat until button is white. Make cloudy cell suspension. Drop 2-3 drops on precleaned, cold, wet slides and air dry. III. Chromosome Banding Analysis: It should be emphasized that all analytical and recording procedure are in accordance with the Southeastern Cancer Study Group Protocol (see attached). Trypsin-giemsa or quinacrine banding is employed to identify the chromosomes. The first 20 cells encountered are analyzed without selection for the quality of the metaphase. This procedure is necessary to avoid laboratory bias because chromosomes from leukemic cells have fuzzier morphology than the chromosomes from normal cells prepared in the same marrow, from these metaphases the number and type of abnormalities in the leukemic cells are identified. The numerical and structural anomalies of each cell studies are noted in the analysis sheet. Photographs of at least 3 cells are taken if all cells are normal. When an abnormal cell is observed it is photographed. For each abnormal cell line at least two cells are photographed. IV. Karyotyping Two karyotypes of normal cells and at least one for every abnormal cell line is prepared according to the International System for Cytogenetic nomenclature (1985). V. Reporting: Copies of mounted karyotypes of normal and abnormal cell lines are sent to the referring physician. Included in this report are the number of cells analyzed and the number of karytypes prepared. Numerical and structural correlation and the prognostic significance are provided with appropriate references. It should be noted that all studies are done under the supervision and are reviewed by multiple physicians certified by the American Board of Medical Genetics. Furthermore, the Director of the clinical cytogenetics laboratory is on the Review and Quality Control Committee of the Southeast Regional Genetics Group (Maternal Child Health Region VIII). For quality assurance procedures: Should results of worksheets not be reproducible and this can be proven by the Government; the Government may terminate this contract with the contractor. The effective termination date will be the date of termination. The contractor will only be paid for test performed up to the date of termination.
- Place of Performance
- Address: Tennessee Valley Healthcare;Alvin C. York Campus;3400 Lebanon Pike;Murfreesboro, TN
- Zip Code: 37129
- Country: United States
- Zip Code: 37129
- Record
- SN01143521-W 20060916/060914220724 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
| FSG Index | This Issue's Index | Today's FBO Daily Index Page |