SOLICITATION NOTICE
66 -- Applied Biosystems BigDye Terminator Reagent
- Notice Date
- 3/3/2011
- Notice Type
- Presolicitation
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Office of Acquisitions, 6120 Executive Blvd., EPS Suite 600, Rockville, Maryland, 20852
- ZIP Code
- 20852
- Solicitation Number
- NCI-110041-MW
- Archive Date
- 4/1/2011
- Point of Contact
- Melissa R. Ward, Phone: 301-402-4509, Caren N Rasmussen, Phone: (301) 402-4509
- E-Mail Address
-
wardmr@mail.nih.gov, cr214i@nih.gov
(wardmr@mail.nih.gov, cr214i@nih.gov)
- Small Business Set-Aside
- N/A
- Description
- Contracting Office Address Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Office of Acquisitions, 6120 Executive Boulevard, EPS Suite 600, Room 6072, Rockville, MD 20852, UNITED STATES. Description The National Cancer Institute (NCI), Division of Cancer Epidemiology and Genetics (DCEG), plans to procure the brand name Applied Biosystems BigDye Terminator Reagent, 5,000 reactions manufactured by Applied Biosystems, 850 Lincoln Centre Dr., Foster City, CA 94404. This acquisition will be processed in accordance with simplified acquisition procedures as stated in FAR Part 13.106-1(b)(1). The North American Industry Classification System Code is 334516 and the business size standard in 500. Only one award will be made as a result of this solicitation. This will be awarded as a firm fixed price type contract. Delivery: 1 week after receipt of purchase order The goal of the Laboratory of Translational Genomics is to dissect the genetic basis of single nucleotide polymorphisms (SNPs) validated in large scale, genome-wide association studies (GWAS). This approach will identify new regions to be studied in great detail and eventually applied to large molecular epidemiology studies to estimate the magnitude of the effect. To accomplish this goal, thousands of patient DNA samples are sequenced each year to identify common gene variants, known as single nucleotide polymorphisms (SNPs). This genotyping is done using a well-established, high-throughput sequencing platform that utilizes BigDye® Terminator version 3.1 (BDT) cycle sequencing chemistry. The contractor shall provide one TF,BDT v3.1 RR-5000 & SEQ BUFFER Facts and Reasons to Justify Other than Full and Open Competition 1. Statutory authority: Statute: This acquisition is conducted under the authority of section 4202 of the Clinger-Cohen Act of 1996 as set forth in 13.106-1 (b)(1). (b) Soliciting from a single source. (1) For purchases not exceeding the simplified acquisition threshold, contracting officers may solicit from one source if the contracting officer determines that the circumstances of the contract action deem only one source reasonably available (e.g., urgency, exclusive licensing agreements, or industrial mobilization). No other requirement and/or type for supply or service will satisfy agency need. In order to continue the laboratory's mission of validating genetic variants that have been found to be associated with increased risk for certain cancers in GWAS, NCI needs to purchase more cycle sequencing reagents. BDT has been used for over nine years in the laboratory to provide high-accuracy base assignments for comparative sequencing applications, namely SNP identification. NCI receives various types of DNA extracts from all over the world that require sequencing, so it is imperative that NCI's sequencing chemistry performs well on a wide range of template types and qualities. The Applied Biosystems BDT accomplishes this, and it also tackles complex, repetitive sequence patterns that the lab often sees. In addition to the above reasons, BDT chemistry from Applied Biosystems is unique because it is compatible with NCI's existing genotyping platform, a 3730xl DNA Analyzer from Applied Biosystems, along with the data collection and analysis software. The salient characteristics that apply to the BigDye Terminator reagent are: • The reagent must be a ready reaction mix containing dye terminators, deoxynucleoside triphosphates (dNTPs), Taq DNA polymerase, magnesium chloride and reaction buffer for quality control. • The reagent is required to be compatible with our current sequencing platform, 3730xl DNA Analyzer from Applied Biosystems. • The dyes in the reagent must be compatible with "Sequence Analysis Software version 5.2". • The dyes in the reagent must be compatible with "Data Collection Software" version 3.0. • The reagent needs to be formulated to generate long reads, as opposed to short, rapid read sequences. • The reagent must be flexible enough to perform well a variety of templates including PCR products, plasmids and large insert clones. • The reagent must not require an ethanol precipitation step to sequence plasmids. • The reagent is required to be robust enough to perform well with difficult templates such as those with dinucleotide repeats and other challenging sequence motifs. This is not a solicitation for competitive quotations. However, if any interested party believes they can meet the above requirement, they may submit a statement of capabilities. All information furnished must be in writing and must contain sufficient detail to allow the NCI to determine if it can meet the above unique specifications described herein. An original and one copy of the capability statement must be received in the NCI Office of Acquisitions on or before 11:00 AM EST on March 17, 2011. No electronic capability statements will be accepted (i.e. email or fax). An original and one copy must be sent to the NCI Office of Acquisitions at the address stated above. All questions must be in writing and can be faxed (301) 402-4513 or emailed to Melissa Ward, Contract Specialist, atwardmr@mail.nih.gov. A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. In order to receive an award, contractors must have valid registration and certification in the Central Contractor Registration (CCR) www.ccr.gov and the Online Representations and Certifications Applications (ORCA), http://orca.bpn.gov. No collect calls will be accepted. Please reference solicitation number NCI-110041-MW on all correspondence.
- Web Link
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- Record
- SN02392788-W 20110305/110303234727-6a0a736efdb53a16aabc06133f8dfb70 (fbodaily.com)
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