SOLICITATION NOTICE
66 -- Optical Fluorescence Confocal Microscope
- Notice Date
- 8/4/2011
- Notice Type
- Presolicitation
- NAICS
- 333314
— Optical Instrument and Lens Manufacturing
- Contracting Office
- Department of the Navy, Office of Naval Research, Naval Research Laboratory/STENNIS, John C. Stennis Space Center, Building 1008, RM 217, Stennis Space Center, Mississippi, 39529-5004, United States
- ZIP Code
- 39529-5004
- Solicitation Number
- N0017311RWR03
- Archive Date
- 8/4/2012
- Point of Contact
- Ryan Wheelock, Phone: 228-688-5434, Richard D Sewell, Phone: 228-688-4571
- E-Mail Address
-
ryan.wheelock@nrlssc.navy.mil, richard.sewell@nrlssc.navy.mil
(ryan.wheelock@nrlssc.navy.mil, richard.sewell@nrlssc.navy.mil)
- Small Business Set-Aside
- N/A
- Description
- NOTICE OF INTENT TO AWARD SOLE SOURCE PROCUREMENT FOR AN OPTICAL FLUORESCENCE CONFOCAL MICROSCOPE SYSTEM. The Naval Research Laboratory intends to award a contract on the basis of other than full-and-open competition to Nikon Instruments, Inc. for a Nikon Ti-E Inverted Microscope with A1-R Hybrid Resonant Scanner and Laser Total Internal Fluorescence Confocal Microscope (TIRF). The required system shall include an inverted fluorescence confocal microscope with differential interference contrast (DIC), laser total internal reflection fluorescence (TIRF), point scanning confocal and high speed resonant scanning confocal microscopy to monitor and quantitate single molecule/ protein fluorescence in nanofluidic structures. The DIC shall function with both a provided UV/Vis lamp, as well as in conjunction with the confocal microscope. The required system shall incorporate both TIRF and scanning confocal microscopy in the same instrument to perform single molecule/protein fluorescence detection at positions both within 200 nm of a coverslip and as much as 10 microns removed from the coverslip. For enhanced sensitivity and light collection efficiency, the laser TIRF system shall incorporate an EMCCD detector and use a 100x objective (oil) with numerical aperture of 1.49 or better. The required system shall incorporate a point scanning confocal to enable high resolution imaging and a resonant scanning confocal to enable high speed imaging. The point scanning confocal shall provide high resolution imaging (4096 x 4096 pixels or better) with scanning speeds of 2 frames per second or faster for 512 x 512 pixels, while a resonant scanning confocal will enable high speed imaging (30 frames per second or faster for 512 x 512 pixels; 420 frames per second or faster for 512 x 32 pixels; 15,600 lines/sec or faster line speed). The required system shall possess laser diodes at two colors (488 nm and 561 nm) with 100mW in power of intensity for each line to ensure sufficient photon flux for single molecule fluorescence. The confocal microscope shall be able to perform two color experiments with the two lasers, simultaneously, and be expandable to four lasers. Four photomultiplier tube detectors shall be supplied with the confocal system, each of which can be operated simultaneously or in sequential mode. The required system shall possess real-time drift correction capability over hours to days with a motorized nosepiece in order to eliminate focus drift in time-lapse observations cause by room temperature variations or by the addition of reagents to a sample. Because of the extreme sensitivity requirements for this microscope to attain single molecule detection, the required system shall have hexagonal pinholes instead of a continuous variable square pinhole. Higher brightness equivalent to that of an ideal circular pinhole is achieved while maintaining the confocality and realizing 30% more light. The required system shall incorporate a low angle incidence method with the dichroic mirrors rather than the conventional 45 degree incidence angle method for dichroic mirrors, in order to realize a 30% increase in fluorescence efficiency. The Nikon Instruments, Inc Ti-E Inverted Microscope with A1-R Hybrid Resonant Scanner and Laser Total Internal Fluorescence Confocal Microscope (TIRF) is the only system available that can meet the Government’s requirements stated above. Vendors who feel capable of providing the supplies stated above shall fully identify their interests and submit written data or other verifiable documentation that demonstrates conclusively the capabilities of their proposed equipment to meet the stated requirements within 15 days after publication of this synopsis. Responses received after 15 days or without the required documentation will be determined non-responsive to the synopsis, and will not be considered.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/DON/ONR/Code3235/N0017311RWR03/listing.html)
- Place of Performance
- Address: 4555 Overlook Ave., SW Washington, District of Columbia, 20375, United States
- Zip Code: 20375
- Zip Code: 20375
- Record
- SN02522921-W 20110806/110805000007-05aa915544253f759c336e115935bcd7 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
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