SPECIAL NOTICE
66 -- Fluidigm Access Array 2AX +FC1 System
- Notice Date
- 8/10/2012
- Notice Type
- Special Notice
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Agriculture, Animal and Plant Health Inspection Service, Administrative Services Division/Contracting, 100 North 6TH Street, Butler Square 5TH Floor, Minneapolis, Minnesota, 55403
- ZIP Code
- 55403
- Solicitation Number
- APHIS-PR-12-598762
- Archive Date
- 9/27/2012
- Point of Contact
- Carol R. Dingess, Phone: 612-336-3208
- E-Mail Address
-
carol.dingess@aphis.usda.gov
(carol.dingess@aphis.usda.gov)
- Small Business Set-Aside
- Total Small Business
- Description
- The United States Department of Agriculture (USDA)/Animal Plant Health Inspection Service (APHIS)/Plant Protection and Quarantine (PPQ) Facility in Edinburg, Texas intends to negotiate a sole source Purchase to the Fluidigm Corporation for an Access Array 2AX + FC1 System. Description of Supplies : Fluidigm Access Array 2AX +FC1 System 85,311.00 Basic Application and Training 5,535.00 2 - IFC Controller AX Service Plans 7,066.00 FCI Cycler Service Plan 1,518.00 Materials Laboratory reagents and IFCs 450.00 Total $99,880.00 This procurement is being conducted in accordance with FAR 6.302-1 The property or services needed are available from only one responsible qualified source at this time and no other type of property or services will satisfy these needs. The Fluidigm Access Array 2AX +FC1 System is a unique fully integrated system enabling sample capture and library preparation (concentrating DNA regions) for next generation sequencing using an1plicon tagging and long range PCR on an Access Anay Integrated Fluidic Circuit (IFC). APHIS/PPQ has found that other similar instrumentation that construct libraries may also perform sample capture and library preparation for genome sequencing. However, amplicon (short DNAs) preparation is solely performed by the Fluidigm system. This is one essential and important difference between the Fluidigm and other similar instruments. Since the methods developed at APHIS/PPQ for pest identification, risk assessment, and pathway analysis do not rely on costly genome sequencing and rather relies on information from short DNAs which can be very informative and cost-effective as compared to the entire genome. Information from short DNAs are not as resource intensive and the protocols used to generate the shorter amplicons are not as complex which is important in our laboratory where many of the end users are technicians that are not seasoned researchers. The system is solely produced by Fluidigm, with the key components patented and designed to operate as an integrated system. No third party systems or components are licensed to use the Fluidigm instrument or Access Array IFCs. The products resulting from the operation of the Fluidigm Access Array instrument are amplicons libraries that can be used on a variety ofNGS instruments, including those from Roche/454, Illumina, Life Technologies, and Pacific Biosciences, making it the only sequencing diagnostic system for target enrichment. The access array allows for the pool of all of the amplicons into single library prep, so individual preps do not need to be performed on each sample or barcode or amplicon thereby reducing further the costs. Unlike similar instruments which cannot multiplex, during amplification on the Fluidigm Access Array, adaptors and barcodes are added to each amplicon using a 4 primer PCR, basically performing the library construction during amplification. This is the strength of the Access Array System for highly multiplexed multiple amplicon sequencing which are important in reducing the time and costs to product delivery, which is not available in the other systems. When all of the products are pooled by the microfluidics array. The result is a single sample with the standard 454 (or illumina or ion torrent) library with standard adaptors that then can be put straight into emulsion PCR for NGS. Unlike other systems, there is no need to do adaptor or barcode ligation (attaching), the process is thereby simplified and costs further reduced. This Notice of Intent is not a request for competitive proposals. No solicitation documents are available and telephone requests will not be honored. However, if a firm believes it can meet the requirements it must furnish information about its product and services, to the Contracting Officer by 4:00pm. Central Time (CT) by August 27, 2012. Supporting evidence must be furnished in sufficient detail to demonstrate the ability to comply with the above requirement. The North American Industry Classification System (NAICS) code for this acquisition is 334516, and the size standard is $ 500 Employees. Responses received after the response date or without the required information will be deemed nonresponsive to the synopsis and will not be considered. Capability statements may be sent to: Carol Dingess, Contracting Officer, USDA-APHIS-MRPBS, ASD Procurement Branch, Butler Square Building, 5th Floor, 100 N. 6th Street, Minneapolis, MN 55403, or via email @ Carol.Dingess@aphis.usda.gov or fax @ (612) 336-3550.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/USDA/APHIS/Contracting/APHIS-PR-12-598762/listing.html)
- Place of Performance
- Address: USDA/APHIS/PPQ/PDDM, Moore Air Base, 22675 N. Moorefield Rd., Bldg 6414, Edinburg, Texas, 78541, United States
- Zip Code: 78541
- Zip Code: 78541
- Record
- SN02834353-W 20120812/120810235336-ea5ff49a7ced65367f8f01f6374e19d4 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
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