SOURCES SOUGHT
B -- PROVIDE ANALYSIS OF SAMPLES FOR LIPID CLASSES AND FATTY ACIDS FOR THE PUPOSE OF DETERMINING THE AMOUNT AND TYPE OF LIPIDS BIOMARKERS IN JUVENILE CRAB AND ARCTIC COD
- Notice Date
- 7/15/2013
- Notice Type
- Sources Sought
- NAICS
- 541690
— Other Scientific and Technical Consulting Services
- Contracting Office
- Department of Commerce, National Oceanic and Atmospheric Administration (NOAA), Western Region Acquisition Division, 7600 Sand Point Way, Northeast, Seattle, Washington, 98115-6349
- ZIP Code
- 98115-6349
- Solicitation Number
- WAD-NFFS7100-13-03626
- Archive Date
- 8/6/2013
- Point of Contact
- Maria L Mendoza, Phone: (206)526-6671
- E-Mail Address
-
Maria.L.Mendoza@noaa.gov
(Maria.L.Mendoza@noaa.gov)
- Small Business Set-Aside
- N/A
- Description
- This is a SOURCES SOUGHT SYNOPSIS and is not a presolicitation announcement. The purpose of this Market Research and Sources Sought Notice is to gain knowledge of potential qualified Small Business, HUBZone, 8(a), Service Disabled Veteran owned, and Women owned Small Business firms. Responses to this Sources Sought Synopsis will be used by the Government in making appropriate acquisition decisions. BACKGROUND: Information of the lipid classes and fatty acids in fish and crabs are required for a number of ecological and essential fish habitat studies. Lipid analyses requires the use of thin layer chromatography with flame ionization detection with a (TLC/FID)MARK V or MARK IV Iatroscan. These are outline for our two years of research listed below: TASK 1: Objectives • To determine differences in the physiological condition of Arctic cod from the nearshore Arctic (warm water) to those collected in colder, offshore areas of the Chukchi Sea. This comparison will provide further insight into whether Arctic cod benefit or are currently stressed in nearshore environments. Fatty acid biomarker analyses will provide information as to whether food sources are qualitatively similar. • To determine the effect of total lipids, food quality, ration, and temperature on the lipid class storage and fatty acid composition of juvenile Tanner crabs. TASK 2: Objective • To determine the interactive effects of food availability and temperature on the growth and lipid storage of Arctic cod. Measures of lipid class storage will provide a biological measure of fish condition under these proposed environmental changes to nearshore Arctic environments. OBJECTIVE: The objective of this agreement is to have the vendor provide analysis of samples for lipid classes and fatty acids for the purpose of determining the amount and type of lipids and biomarkers in juvenile crab and Arctic cod. SCOPE OF WORK: The vendor will provide a Fee Book Service in which an independent contractor will be performing analysis of samples for lipid classes and fatty acids. This analysis will be performed over an approximate 2 ½ yr period of time. TASK 1: A total lipid panel for 107 samples will be completed over the time period specified (DATE OF AWARD-July 31, 2014) Lipid Panel Analyses include: Lipid Class Analysis of Fatty Acids Lipid Extraction Lipid Class Analyses Comprehensive lipid profiles as % of total lipid and by dry weight will be electronically supplied in a series of Excel spreadsheets. TASK 2: An extraction and analyses of lipid classes for 164 samples over the time period specified (from date of award -July. 31, 2015). Lipid Panel Analyses include: Lipid Extraction Lipid Class Analyses Additional time is provided so that contractor can provide the service and data requirements requested (August 1, 2015- July 31, 2017) PERIOD OF PERFORMANCE: The period of performance for this contract shall be from date of award through Jan 31, 2016. CONTRACTOR PERFORMANCE REQUIREMENTS: Lipid Analysis: Lipids from Arctic cod and juvenile Tanner crabs will be extracted in chloroform/methanol using a modified Folch procedure. Lipid classes were determined using thin layer chromatography with flame ionization detection (TLC/FID) with a MARK V Iatroscan (Iatron Laboratories, Tokyo, Japan). Extracts will be spotted on silica gel coated Chromarods and a three stage development system will be used to separate lipid classes. The first separation consists of 20-min developments in 99:1:0.05 hexane:diethyl ether:formic acid. The second separation consists of a 40-min development in 80:20:1 hexane:diethyl ether:formic acid. The last separation consists of 15-min developments in 100% acetone followed by 10-min developments in 5:4:1 chloroform: methanol:water. After each separation, the rods will be scanned and the three chromatograms combined using T-data scan software (RSS Inc., Bemis, TN, USA). The signal detected in millivolts will be quantified using lipid standards (Sigma, St Louis, MO, USA). Lipid classes will be expressed both in relative (mg g-1 wet weight) and absolute amounts (mg animal 1). Fatty Acid Analysis: Total lipids from juvenile crab and plankton will also be analyzed for fatty acid composition. Fatty acid methyl esters (FAME) will be prepared by transesterification with 10% BF3 in methanol at 85 C for 1 h. A Varian model 3400 GC equipped with a Varian 8100 autosampler will be used for fatty acid analysis. The column is an Omegawax 320 column, 30 m, 0.32 mm i.d., 0.25 lm film thickness. Hydrogen will be used as the carrier gas and with the flow rate set at 2 ml min-I. Fatty acid peaks will be integrated using Varian Star Chromatography Software (version 4.02) and identification will be made with reference to known standards (PUFA 1 and 37 Component FAME Mix, Supelco Canada, ON). SPECIFIC OBJECTIVES/TASKS: • The contractor will be provided pre-extracted frozen samples from the Fisheries Behavioral Ecology Program in Newport, OR and the contractor will be responsible for storing those samples at -80°C prior to analysis. • The contractor with analyze lipid extracts for fatty acids by GC-FIC and lipid classes by TLC-FIC. PROJECT DELIVERABLES and DEADLINE FOR EACH • TASK 1: July 31, 2015 -The contractor will provide data spreadsheets of quantitative values lipid classes and fatty acids. • TASK 2: Jan. 31, 2016- The contractor will provide data spreadsheets of quantitative values lipid classes and fatty acids. The North American Industry Classification Code (NAICS) is 541690 with a size standard of $14M. Interested firms shall provide, in writing, a brief narrative (no more than 3 pages in length) and documentation to support your firm's capability and experience meeting the requirements herein and include your firm's name, address, point of contact, telephone number, DUNS Number, CCR Cage Number, business type and size, i.e., small, large, non-profit, educational, and so forth. Any response that does not include this information will not be considered. The Government will review your response to determine if your firm has the experience, knowledge and capability to perform the services as specified above. This request for information does not constitute a commitment, implied or otherwise, that a procurement action will be issued. Nor does this request for information constitute a commitment, implied or otherwise that any procurement action that may be issued for the Government's requirements. No entitlement to payment of direct or indirect costs or charges by the Government will arise as a result of the submission of contractor's information. The Government shall not be liable for or suffer any consequential damages for proprietary information not properly identified. Proprietary information will be safeguarded in accordance with the applicable Government regulations. All contractors doing business with the Government are required to be registered with the System for Award Management (SAM). NOTE: THIS SYNOPSIS IS NOT CONSIDERED A REQUEST FOR PROPOSAL BUT A REQUEST FOR INFORMATION ONLY. No solicitation document is available. No contract will be awarded on the basis of responses received to this notice. Detailed information must be submitted by email to the Purchasing Agent, Maria L. Mendoza at maria.l.mendoza@noaa.gov by09:00 AM local Seattle time (PST), on Monday, JULY 22, 2013. Questions of any nature must be addressed in writing as TELEPHONE INQUIRIES WILL NOT BE HONORED.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/DOC/NOAA/WASC/WAD-NFFS7100-13-03626/listing.html)
- Place of Performance
- Address: seattle, Washington, 98115, United States
- Zip Code: 98115
- Zip Code: 98115
- Record
- SN03115944-W 20130717/130715234226-f0f76f4df74e3dc5bf6bb5321de3f5cc (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
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