MODIFICATION
66 -- DNA Sequencing System, Accessories, and Supplies
- Notice Date
- 8/27/2013
- Notice Type
- Modification/Amendment
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, Office of Administration, 6011 Executive Blvd, 5th Floor, Rockville, Maryland, 20852-3804, United States
- ZIP Code
- 20852-3804
- Solicitation Number
- NIHOD2013437
- Point of Contact
- Timothy Johnson, Phone: 301402-5450, Foteni - Tiffany, Phone: 301-402-3079
- E-Mail Address
-
johnsontim@mail.nih.gov, tiffanyf@od.nih.gov
(johnsontim@mail.nih.gov, tiffanyf@od.nih.gov)
- Small Business Set-Aside
- N/A
- Description
- The National Institutes of Health (NIH), Office of the Director, on behalf of the National Institute on Deafness and Other Communication Disorders (NIDCD), intends to solicit and negotiate on a non-competitive basis, with only one source, Illumina, 5200 Illumina Way, San Diego, CA 92122 for a DNA Sequencing System, Accessories, and Supplies. The cited authorities are 41U.S.C. 253(c) (1), as set forth in FAR 6.302-1. The Classification Code has been revised to read as "66 -- Instruments & laboratory equipment". The NAICS Code has been revised to read as "334516 -- Analytical Laboratory Instrument Manufacturing". The National Institute on Deafness and Other Communication Disorders (NIDCD) currently performs genome-wide linkage scans and RNA expression array studies using an Illumina Bead Array Reader. Illumina is the only manufacturer providing a sequencer using clonal cluster generation and bridge amplification. These applications are necessary for producing comparable and consistent prior research results. Continuity and compatibility with historical research data, across a wide range of applications, is an essential requirement for this acquisition. This equipment provides an easily scalable range of research data output; from 10 Gb to 300 GB, per application run, making it highly versatile, supporting a wide range of projects. Interested contractors are invited to submit capability statements. Capability statements must document the following: Government Minimum Requirements: - Illumina HiSeq 1500 Sequencing System, or equal: High-Output Mode Requirements- (Transcription Factor, mRNA-Seq, TruSeq Exome Seq, Human Whole Genome) - Read Length of 1 x 36, Single Flow Cell of 47 to 52 Gb (1.5 billion reads), yields results in 2 days - Read Length of 2 x 50, Single Flow Cell of 135 to 150 Gb (1.5 billion reads), yields results in 5.5 days - Read Length of 2 x 100, Single Flow Cell of 270 to 300 Gb (1.5 billion reads), yields results in 11 days Quality Requirements: Greater than or equal to 85% above Q30 at 2 x 50 bp or 80% of bases above Q30 at 2 x 100bp Rapid-Run Mode Requirements - (Transcription Factor, mRNA-Seq, TruSeq Exome Seq, Human Whole Genome, De Novo Sequencing) - Read Length of 1 x 36, Single Flow Cell of 9 to 11 Gb (300 million reads), yields results in 7 hours - Read Length of 2 x 50, Single Flow Cell of 25 to 30 Gb (300 million reads), yields results in 16 hours - Read Length of 2 x 100, Single Flow Cell of 50 to 60 Gb (300 million reads), yields results in 27 hours - Read Length of 2 x 150, Single Flow Cell of 75 to 90 Gb (300 million reads), yields results in 40 hours Quality Requirements: Greater than or equal to 85% above Q30 at 2 x 50 bp, 80% of bases above Q30 at 2 x 100 bp, or 75% of bases above Q30 at 2 x 150 bp - iScan System, or equal requirements (per BeadChip, listed): Approx. Scan Time Per Sample (minutes) & Manual/Automation (per week) Infinium HumanOmni5 - 26 128/272 Infinium HumanOmni2.5-8 - 11.4 256/608 Infinium HumanOmniExpress+ - 7.5 256/960 Infinium Methylation450K - 5.0 384/1,440 Infinium HumanCytoSNP-12 - 2.9 384/1,728 Infinium HD iSelect (12x1) - 2.9 384/1,728 Infinium HD iSelect (24x1) - 1.0 768/3,456 BovineLD - 0.3 768/3,456 Universal-32 BeadChip for GoldenGate Genotyping- 0.3 1,440/1,440 - MiSeq (Benchtop Sequencing) System, or equal requirements: - 2 x 300 paired-end reads and up to 15 Gb of data in a single run. - Access to widely-published and accurate sequencing chemistry, backed by > 3,800 peer-reviewed publications. - Multiplex up to 96 samples per run. - Perform accurate bi-directional amplicon sequencing. - Generate complete D e Novo assemblies. - Targeted Resequencing: Amplicon Sequencing (tens of targets @ 1 x 250, read length), Amplicon Sequencing (hundreds of targets @ 2 x 250, read length), Hybrid Capture (thousands of targets @ 2 x 75, read length), 16S Metagenomics @ 2 x 150, Clone Checking @ 1 x 36. - Small-Genome Sequencing: De Novo @ 1 x 36/ 2 x 250, Resequencing @ 1 x 36/ 2 x 250, Plasmids @ 1 x 36/ 2 x 250. - RNA Sequencing: Small RNA Sequencing @ 1 x 36, RNA-Seq (microbial) @ 2 x 50-250. - Quality Control: Library QC @ 2 x 25. - Regulation: ChIP-Seq @ 1 x 36. Delivery and Installation, Included. The government requires delivery to made within 90 days from the date the contractor receives an order. All responsible sources may submit a capability statement, proposal, or quotation, which shall be considered by the agency. Respondents to this notice shall provide capability statements (one original and three (3) copies) no later than 5:00 p.m. EST on September 4, 2013 to Timothy Johnson (Ref. NIHOD2013437), National Institutes of Health, Office of Acquisitions, 6011 Executive Blvd., Room 529W (MSC 7663) Bethesda, MD 20892-7663. PLEASE NOTE: If you are using a courier service, Federal Express, or UPS, the city, state, and zip code should read ROCKVILLE, MD 20852). Please be aware that the U.S. Postal Service's "Express Mail" DOES NOT deliver to the Rockville, Maryland address AND delivery to the Bethesda, Maryland address will result in a delayed delivery to our office in Rockville, MD (up to 3 - 5 days). Email and facsimile copies will not be accepted. Disclaimer: This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Respondents are advised that the Government is under no obligation to acknowledge receipt of information received or provide feedback to respondents with respect to any information submitted.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/OoA/NIHOD2013437/listing.html)
- Place of Performance
- Address: National Institutes of Health, Bethesda, Maryland, 20892, United States
- Zip Code: 20892
- Zip Code: 20892
- Record
- SN03163956-W 20130829/130827235443-1584851f23a006453253473db3d33067 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
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