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FBO DAILY - FEDBIZOPPS ISSUE OF FEBRUARY 13, 2015 FBO #4829
DOCUMENT

65 -- LightSheet Illumination System - Attachment

Notice Date

2/11/2015
 

Notice Type

Attachment
 

NAICS

333314 — Optical Instrument and Lens Manufacturing
 

Contracting Office

Department of Veterans Affairs;Program Contract Organization East(PCOE);1100 1st Street, 7th Floor, Suite 732;Washington DC 20002
 

ZIP Code

20002
 

Solicitation Number

VA26815Q0037
 

Response Due

2/19/2015
 

Archive Date

2/19/2015
 

Point of Contact

Elaine F DiBucci
 

E-Mail Address

2-3432<br
 

Small Business Set-Aside

N/A
 

Description

The Ralph H. Johnson VAMC, Charleston, SC, Research Department has a requirement to purchase Light Illumination System/equipment. This is not a solicitation announcement. This is a sources sought synopsis only. The purpose of this synopsis is to gain knowledge of potential qualified sources and their size classification (large business, small business, 8(a), SDVOSB, etc.), relative to NAICS code 333314 (size standard of 500 employees). Responses to this synopsis will be used by the Government to make appropriate acquisition decisions. Based on responses to this synopsis, the Government may issue this RFQ as a Brand Name or Equal requirement in accordance with VAAR Clause 852.211-73, with Zeiss as the Brand Name Manufacturer. The System includes the following specifications: System Description and Use Multiview Light Sheet Fluorescence Microscope to performs fluorescence imaging on large, living samples, with virtually no phototoxicity or bleaching and with high temporal resolution which allows researchers to record the development of large, living samples and gently image them to deliver exceptionally high information content. With a focus on advancing an area of clinical investigation centered on pathology application of the technology will address areas of pathology that cannot be addressed using current technology applied in clinical practice and will provide substantial improvements on current practice. The clinical use will be on the development of new diagnostics and biomarkers and provide new insight into the pathophysiology of disease. Clinical applications include Multiview Light Sheet Fluorescence Microscope: 1.The state-of-the-art microscopy platform (fluorescent lightsheet microscopy) will enable the rapid acquisition of high-resolution three-dimensional (3D) images directly from tissue samples up to several millimeters in thickness. The primary aim of the instrument will be to determine the ability to perform fluorescent lightsheet microscopy directly on archival patient tissue samples and to use the method to identify 3D morphological hallmarks of disease. 2.The growth of new blood vessels is fundamentally a three-dimensional problem, where cells move and connect throughout the tissue to develop an extensive tubular network. To date no one has imaged this process in its full three dimensions throughout the different layers of eye tissue, as deep imaging techniques were previously unavailable. By comparing diseased and normal tissues using light sheet microscopy, this study will help unravel how and why vessel growth degenerates in retinopathy conditions and provide a new testbed to investigate the effects of new therapeutic targets. 3.Pathologists render a diagnosis by reading thin tissue sections mounted on glass slides. An ability to infer three-dimensional (3D) structure from two-dimensional (2D) tissue sections is crucial for diagnosis and for full characterization of many disease processes, but this depends on the skill and experience of the individual pathologist. With advances in digital methods (lightsheet technology), it will now be feasible to have a new workflow in which pathologists are presented with aligned whole slide images (WSIs) of serial sections. The expectation is for faster evaluation, greater objectivity, and improved diagnostic accuracy. 4.The clinical use for the rapid 3D imaging of biopsies to determine the diagnosis for patients is a significant advance in the field. The system will provide a clinical assessment of the patient and provide a unique opportunity for the continual, ongoing developments in the clinical laboratories to support the value of this cutting edge technology to improve the clinical care that can be offered to our veterans. Clinical applications system that can see inside post-mortem tissue nclude: 1.This request is to install system that can see inside post-mortem tissue. Until now researchers have had to slice the tissue into thin sections and then visualize small areas of the tissue the system will enable the entire tissue, such as a whole brain, spinal cord, or heart, is made transparent allowing researchers to investigate the subcellular structure, neuronal network, specific proteins, biomolecular complexes and gene expression profiling at the resolution of single cells. This will enable the researchers to visualize the entire brain, spinal cord, or other tissue for defects associated with neurodegenerative diseases, traumatic brain injury, aging, cancer and stroke. MRI and other imaging methods are good for living tissue, but they do not provide resolution at the single cell level, they cannot identify mild traumatic brain injury, nor can they identify changes that precede full-blown pathology. MRI is not applicable to small animals, such as rats and mice, the animals of choice in neuroscience research. 2.The procured system must have multiphoton microscope and supporting software that can provide the necessary reproducibility, quantification, resolution and throughput to image the cells. This system must provide increased optical resolution and contrast, and also provides for the reconstruction of the cells from the images. Such a microscope also targets the beam of light to a specific point and controls depth (z-axis) of penetration of the laser. The microscope is a high resolution imaging system coupled to real-time downloading to peripheral computer workstations so as to provide maximal throughput. There are currently no microscopes at this station to implement such a system. A dedicated system that has been fashioned to implement is necessary. 3.This integrated equipment must be turn-key and user-friendly. This allows investigators to operate the equipment on their own. Each investigator will be given initial training by the manufacturer on the use of the microscope and camera, and its capabilities. The vendor must be able to supply instrumentation to meet the following specifications: a.A scanned light sheet is used for homogeneous illumination of the specimen. b.A pivot scanner is available for shadow reduction. c.An optional dual side illumination system is available. d.Stand alone, sealed turnkey, laser safe with class 1, eyepiece-free system. e.Environmental control via temperature with or without CO2 incubation. For the heating block, it is controlled using the TempModule S1. With Peltier block, an internal sample chamber temperature sensor is used with the TempModule S1 and TempModule CZ-LSFM. A heating device for humidity is available. f.Up to 6 excitation lines across the entire visible spectrum. All lasers are class 3B. The installed system as a whole is laser class 1. g.Light sheet thickness for the 488nm laser can be adjusted from 2 ?m to approx. 16 ?m depending on the sample. h.Collimator-free illumination beam path due to optimized color correction of the system. i.Embedded specimen size can range from less than 1 ?m up to 5 mm with ability to do a 360 ° turn of the sample. j.Sample mounting utilizes a sturdy, dedicated sample holder and sample chamber for accommodating living specimens. Sample chamber can be cleaned and sterilized. k.Sample chamber and optics are designed for aqueous media (Refractive Index = 1.33) which is ideal for samples requiring incubation media. l.Sample chamber, sample holder, and consumables come within a starter kit. All consumables are available for re-ordering to replenish the starter kit. m.Defined upgrade pathway for upgrading from a single side illumination system to a dual side illumination system with integrated control software. n.A light sheet illumination system utilizing a horizontal microscope setup. o.Overview camera with white light LED for coarse sample positioning. p.Transmission contrast using an IR LED illumination for deeper sample penetration to the detection module when doing fine sample positioning. q.Set-up of the optical beam path is completely motorized and can be configured using the software. r.Four-axis multi-coordinate stage with stepper motors for x /y /z and rotation (?) for multidimensional sample positioning and acquisition along with an Ergo Drive Control Panel and software Specimen Navigator for ease of use. s.Dual camera port for simultaneous 2 channel detection. Up to two detection modules of the same type can be connected to the system. t.Sample mounting from above via a sturdy dedicated sample holder u.Single side illumination or Dual side illumination with Pivot scan (for shadow reduction) available for image acquisition. ? Item #Description/Part Number *Qty 1LightSheet Z.1 Illumination System41090040010010001 2Laser 405nm 50mW40060090110000001 3Laser 445nm 40mW40060090300000001 4Laser 488nm 100mW40060090600000001 5Laser 515nm 50mW40060090800000001 6Laser 561nm 75mW40060091100000001 7Laser 638nm 75mW40060091210000001 8LightSheet Z.1 detection module PCO.Edge40010090300000002 9Liquid Cooling system LCS-BU (D)5453620001 10LightSheet Z.1 Detection optics 20x/1.040010095100000001 11LightSheet Z.1 Detection optics 50x/0.1640010095000000001 12Illulmination Optics LSFM 5x/0.140090090100000002 13ZEN desk 2012 Hardware License Key41013510041200001 14ZEN Module 2014 Lightsheet.Z1 Proc. HWL41013610591300001 15ZEN Module 3D VisArt Hardware Lic. Key41013610241100001 16Storage/Analysis PC Lightsheet Z.1-32TB20506850001 17Monitor TFT 30" HP Z30i41035030040000001 18Filter Module LBF 405/488/56140490091000000001 19Filter Module LBF 405/488/561/63840490091010000001 20Filter Module LBF 445/514/63840490091040000001 21Filter Module Lightsheet Z.1 - DAPI-GFP40490093100000001 22Filter Module Lightsheet Z.1 - CFP-YFP40490093130000001 23Filter Module LSFM - GFP-mCherry40490093170000001 24Filter Module Lightsheet Z.1 - GFP-DRAQ540490093180000001 25Filter Module Lightsheet Z.1 - Cy3-DRAQ540490093220000001 26Filter Module LSFM - CFP-mCherry40490093150000001 27Filter Module Lightsheet Z.1 - GFP-Cy340490093160000001 28TempModule CZ-LSFM with accessories17905460001 29CO2-Module Lightsheet Z.117905480001 30Temp Module S141186090100000001 31Microfilter for sterile CO2 Incubation5479120001 32Antiseptic for Peltier-Cooling (O)5581300001 33Heating Device Humidity S1 (D)41185790500000001 34Heatingblock Sample Chamber LS Z.119079840001 35Peltierblock Sample Chamber LS Z.119079850001 36Temperature Sensor Lightsheet Z.120030560001 37Table f/LSM 710/780/880 30x36, SP, I-32541084329548120001 38Indimo: Clearing ready LSFM41090021071460001 39INDIMO Sample Chamber and Clarity Objective41090021049531 40Storage/Analysis PC Lightsheet Z.1-32TB20506850001 41Memory Upgrade (24GB) f/Data Analysis PC20014450002 42Monitor TFT 30" HP Z30i41035030040000001 43ZEN desk 2012 Hardware License Key41013510041200001 44ZEN Module 2014 Lightsheet.Z1 Proc. HWL41013610591300001 45ZEN Module Deconvolution HW License Key41013610211100001 46ZEN Module 3D VisArt Hardware Lic. Key41013610241100001 47Arivis 3D/4D softwareAri1 48Trigger adapter & cable f. Lightsheet Z.20643080001 Delivery Information: This notice is not a request for competitive proposals, however; any responsible source who believes it is capable of meeting the requirement may submit a capability statement to the contracting office no later than Wednesday, February 18, 2015, 4:00PM, EST. Interest/capability statements may be sent to Elaine DiBucci at elaine.dibucci@va.gov and should include company name, address, point of contact, business size, and product information. No telephone responses will be accepted. This notice is to assist the Government in determining sources only. A solicitation is not currently available. If a solicitation is issued, all interested parties must respond to that solicitation announcement separately from the responses to this announcement.
 

Web Link

FBO.gov Permalink
(https://www.fbo.gov/notices/a0503f2ce87f268aca07ef8599c1d5a7)
 

Document(s)

Attachment
 

File Name: VA268-15-Q-0037 VA268-15-Q-0037.docx (https://www.vendorportal.ecms.va.gov/FBODocumentServer/DocumentServer.aspx?DocumentId=1860983&FileName=VA268-15-Q-0037-000.docx)

Link: https://www.vendorportal.ecms.va.gov/FBODocumentServer/DocumentServer.aspx?DocumentId=1860983&FileName=VA268-15-Q-0037-000.docx

 

Note: If links are broken, refer to Point of Contact above or contact the FBO Help Desk at 877-472-3779.
 

Record

SN03640095-W 20150213/150211234905-a0503f2ce87f268aca07ef8599c1d5a7 (fbodaily.com)
 

Source

FedBizOpps Link to This Notice
(may not be valid after Archive Date)

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