SOLICITATION NOTICE
B -- Continuation Study on characterizing knock-out and knock-in zebrafish lines produced
- Notice Date
- 2/25/2015
- Notice Type
- Presolicitation
- NAICS
- 541712
— Research and Development in the Physical, Engineering, and Life Sciences (except Biotechnology)
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Heart, Lung and Blood Institute, Rockledge Dr. Bethesda, MD, Office of Acquisitions, 6701 Rockledge Dr RKL2/6100 MSC 7902, Bethesda, Maryland, 20892-7902
- ZIP Code
- 20892-7902
- Solicitation Number
- HHS-NIH-NHLBI-CSB-(HG)-2015-076-DLM
- Archive Date
- 3/19/2015
- Point of Contact
- Dorothy Maxwell, Phone: 301-435-0352
- E-Mail Address
-
maxwelld@mail.nih.gov
(maxwelld@mail.nih.gov)
- Small Business Set-Aside
- N/A
- Description
- INTRODUCTION: THIS IS A PRE-SOLICITATION NON-COMPETITIVE (NOTICE OF INTENT) SYNOPSIS TO AWARD A PURCHASE ORDER WITHOUT PROVIDING FOR FULL OR OPEN COMPETITION (INCLUDING BRAND-NAME). THIS IS A NOTICE OF INTENT, NOT A REQUEST FOR A PROPOSAL. A SOLICITATION DOCUMENT WILL NOT BE ISSUED AND PROPOSALS WILL NOT BE REQUESTED. The National Heart, Lung, and Blood Institute (NHLBI) Office of Acquisition (OA) on behalf of the National Human Genome Research Institute, (NHGRI), intends to negotiate and award a purchase order on a non-competitive sole source basis to the University of California, Los Angeles, 11000 Kinross Avenue, Suite 211, Box 951406, Los Angeles, California 90095 to provide Phase 2 characterizing knock-out and knock-in zebrafish lines produced. Follow-On Requirement Background : The National Institute of Health (NIH) is the nation's leading medical research agency and the primary Federal agency seeking fundamental knowledge about the nature and behavior of living systems and the application of that knowledge to enhance health, lengthen life, and reduce illness and disability. The National Institutes of Health Undiagnosed Disease Program (UDP) is a trans-National Institute of Health (NIH) initiative that focuses on the most puzzling medical cases referred to the NIH Clinical Center in Bethesda, MD. Many medical specialties from other NIH research centers and institutes contribute the expertise needed to conduct the program, including endocrinology, immunology, oncology, dermatology, dentistry, cardiology and genetics, among the dozens of participating senior attending physicians. A longstanding medical condition that eludes diagnosis by a referring physician can be considered undiagnosed and may be of interest to this clinical research program. The goals of the UDP include finding accurate diagnoses and discovering new diseases that provide insight into human physiology and genetics. The UDP was established to evaluate participants who have undiagnosed medical conditions. Purpose and Objectives : Provide TALEN mediated knock-in zebrafish models containing specific mutations in order to recapitulate disease phenotype as seen in UDP patients. Also generate four constructs of human twist 2 inducible transgenes and produce stable transgenic lines. The laboratory of Dr. Shuo Lin from UCLA has developed a novel TALEN mediated homologous recombination technology that allows for knocking in specific mutations variants that have been seen in UDP patients. These specific zebrafish models support the research needs of the UDP through generating a model organism that can then lead to the characterization of causes of new and rare diseases. The Contractor shall will analyze and phenotypically characterize the germline transmission of zebrafish carrying twist2 and Zac1 knock-in mutations as well as the knockout lines of twist1a, 1b and 3. Overall, they will provide the characterization and analysis of zebrafish knock-in or knock-out lines for 12 genetic loci including 5 from the first contracted year and 7 new genes using the novel TALEN mediated homologous recombination technology that they have developed. The goals of the UDP include finding accurate diagnoses and discovering new diseases that provide insight into human physiology and genetics. The Contractor shall provide functional analysis of mutated genes carried by UDP patients. This will assist the NIH UDP in determining the causality of the patient disorders. Scope of Work : The Contractor shall provide a robust methodology for introducing mutations in zebrafish in order to characterize the potential causality of mutations identified in UDP patients. The Contractor shall provide the following to the UDP laboratory: • Generate and screen missense mutations implicated in human diseases in zebrafish. This will be accomplished by using a TALEN mediated homologous recombination technology that allows knock-in of the specific mis-sense mutations or the CRISPR/Cas9 method to facilitate homologous recombination in zebrafish. • Generate germline transmission of the mutation of interest and proven homologous recombination by methods including both PCR and Southern blotting. • Provide quantitative phenotypic analyses of zebrafish for features of the human disease of interest. • A turn-around-time (TOT) of 52 weeks or less per 12 genes of interest. • Real time free and full sharing of all data with the UDP and its collaborators. Recording of data in the UDP laboratory notebook and database. • A proven track record of at least 20 years of genetic and transgenic analysis of zebrafish. • Co-publication of results with the NIH UDP. • The potential to extend the studies to define disease mechanisms and identify potential therapeutic interventions. A. Desired Results/Functions/End Items: 1. Test results with qualified interpretations in a timely way given the requirements of the test provided. 2. Research collaborations will be conducted via Videoconferences, email communications, shared professional presentations, posters and publications. Grand Round presentations at the Contractor's location and the NIH Clinical Center may also occur. B. Technical Specifications/Standards/Methods: 1. The Contractor shall supply mutant fish sperm or mutant embryos to the UDP for phenotypic analysis. 2. The Contractor shall supply results with qualified interpretations in formats appropriate to the process management systems and for facilitation of other collaborations. C. Special Handling or Handling Requirements: 1. The Contractor shall provide result reports and record methods and materials as appropriate to the highest scientific standards. These materials, methods and results will be shared will agreed upon UDP collaborators. D. Government Responsibilities: 1. The Government will not furnish any government property to the vendor 2. The Government will provide the names and mutations of the genes of interest to the Contractor. 3. The Government will not furnish any facilities to be used by the vendor. E. The Government will be responsible for reviewing and approving reports and similar matters generated: 1. Results will be provided electronically to the UDP via secure email, the UDP ftp site or direct deposition into the UDP process management system. 2. Results will also be shared with UDP collaborators as defined mutually. F. Reporting Requirements and Deliverables: 1. The Government will receive all electronic results and biological materials within 52 weeks of notification for each twelve genes of interest. 2. The Government will receive additional reports for any test added-on after the Contractor receives the original specimen. Period of Performance : 12-Months from Date of Award Regulatory Authority : This acquisition is conducted under the authority of the Federal Acquisition Regulations (FAR) Subpart 13.106-1(b) Soliciting from a single source, only one responsible source and no other supplies or services will satisfy agency requirements. Additional Information : Industry Classification (NAICS) Code is 541712, Research and Development in the Physical, Engineering, and Life Sciences (except biotechnology) and small business size standard is 500. The acquisition is being conducted under FAR Part 13, simplified acquisition procedures, therefore the requirements of FAR Part 6 B Competitive Requirements are not applicable (FAR Part 6.001). The resultant Contract will include all applicable provisions and clauses in effect through the Federal Acquisition Circular (FAC) 05-7 (December 26, 2014). This requirement is under the Simplified Acquisition Threshold of $150,000.00. This synopsis is not a request for competitive proposals. However, interested parties may identify their interest and capability to respond to this notice. Responses to this notice shall contain sufficient information to establish the interested parties' bona-fide capabilities for fulfilling the requirement and include: unit price, list price, shipping and handling costs, the delivery period after contract award, the prompt payment discount terms, the F.O.B. Point (Destination or Origin), the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov." A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. The information received will normally be considered solely for the purposes of determining whether to proceed on a non-competitive basis or to conduct a competitive procurement. All responses must be received March 4, 2015 and must reference synopsis number HHS-NIH-NHLBI-CSB-(HG)-2015-076-DLM, may be submitted to the National, Heart, Lung and Blood Institute, Office of Acquisition, COAC Services Branch, 6701 Rockledge Drive, Suite 6149, Bethesda, Maryland 20892-7902, Attention: Dorothy Maxwell. Response may be submitted electronically to maxwelld@mail.nih.gov. Faxes will not be accepted. Responses will only be accepted if dated and signed by an authorized company representative. "All responsible sources may submit a bid, proposal, or quotation which shall be considered by the agency."
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NHLBI/HHS-NIH-NHLBI-CSB-(HG)-2015-076-DLM/listing.html)
- Place of Performance
- Address: NIH/NHGRI/Undiagnosed Diseases Program, Fishers Lane, Rockville, Maryland, 20852, United States
- Zip Code: 20852
- Zip Code: 20852
- Record
- SN03651808-W 20150227/150225234916-4e5b902ca2eda25531a3d1a5b47016d4 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
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