SOURCES SOUGHT
B -- mg Quantities of Genomic DNA from Bacterial Isolates
- Notice Date
- 3/3/2016
- Notice Type
- Sources Sought
- NAICS
- 621511
— Medical Laboratories
- Contracting Office
- Department of Commerce, National Institute of Standards and Technology (NIST), Acquisition Management Division, 100 Bureau Drive, Building 301, Room B130, Gaithersburg, Maryland, 20899-1410, United States
- ZIP Code
- 20899-1410
- Solicitation Number
- Quantities_of_Genomic_DNA
- Archive Date
- 4/9/2016
- Point of Contact
- Erik Frycklund, Phone: 3019756176, Grace H. Garrity, Phone: 3019752345
- E-Mail Address
-
erik.frycklund@nist.gov, grace.garrity@nist.gov
(erik.frycklund@nist.gov, grace.garrity@nist.gov)
- Small Business Set-Aside
- N/A
- Description
- Sources Sought, mg Quantities of Genomic DNA from Bacterial Isolates This is a SOURCES SOUGHT NOTICE for market research purposes. THIS IS NOT A REQUEST FOR PROPOSALS OR A REQUEST FOR QUOTATIONS. This notice is for planning purposes only and shall not be misunderstood as a Request for Proposal or a Request for Quotation or as an obligation on the part of The National Institute of Standards & Technology (NIST) for conducting a follow-on acquisition. NIST does not intend to award a contract on the basis of this notice, or otherwise pay for the information requested. No entitlement or payment of direct or indirect costs or charges by NIST will arise as a result of submission of responses to this notice and NIST use of such information. The National Institute of Standards and Technology's (NIST's) Biosystems and Biomaterials Division (BBD) provides the measurement science, measurement standards, and measurement technology required to enable world-leading characterization of materials in support of the nation's needs for the determination of the composition, structure, and properties of biomaterials. In partnership with U.S. industry, government agencies and other stakeholders, the Division develops state-of-the-art instrumentation, methods, models and software to accurately and precisely measure biomaterials over a range of length and time scales. The Division provides benchmarking and validation of emerging materials analysis methods, and disseminates reference materials, standards and scientific data to foster innovation and advance a wide range of technologies, such as those for public safety, forensics, homeland security and manufacturing. NIST is seeking responses from all responsible sources, including large, and small businesses. The small business size standard for the applicable NAICS code of 621511 Medical Laboratories - DNA Testing Laboratories is $32.5M. Please include your company's size classification and socio-economic status in any response to this notice. After results of this market research are obtained and analyzed, NIST may conduct a competitive procurement and subsequently award a Purchase Order. If at least two qualified small businesses are identified during this market research stage, then it is anticipated that any competitive procurement that results from this notice will be conducted as a small business set-aside. Additionally, if a qualified 8(a) small disadvantaged business is identified during this market research, NIST may conduct a direct award in accordance with FAR 6.302-5, Circumstances Permitting Other Than Full and Open Competition, Authorized or Required by Statute. The purpose of this notice is to perform market research to understand the baseline cost of generating specific materials. NIST seeks to produce reference materials of purified genomic DNA from bacterial isolates to benchmark next-generation sequencing (NGS) performance. NIST anticipates needing material from 6-25 separate strains that will be selected and supplied by NIST to the contractor. Identity information about the strains will be provided and will not exceed BSL2. These will be organisms that are cultured using documented protocols, such as E. coli, P. aeruginosa, or S. aureus. NIST wishes to produce a maximum number of bacterial isolates' DNA for reference material, but the final number will be determined based on the associated cost. Under a potential contract, the Contractor would be required to provide the following for each isolate: 1) Colony purify each isolate through microbiological plating/streaking 2) Grow/culture liter quantities of each pure isolate in appropriate liquid broth 3) Extract/Isolate/Purify total DNA from each pure culture 4) DNA extraction methods must minimize DNA shearing such that high molecular weight DNA is obtained (average size minimum > 10 kilobases, preferably > 100 kilobases). As demonstrated by gel electrophoresis or Agilent Bioanalyzer or other molecular method(s). 5) A minimum yield of 10 milligrams of pure DNA must be achieved for each culture. 6) Demonstrate DNA purity via spectrophotometry absorbance. A260/A280 >1.8 7) Normalize DNA concentration to 500 ng/µl in ultrapure 1x Tris-EDTA pH=7.4 8) Demonstrate DNA concentration by UV absorbance and Florescence Spectrometry (Qubit) 9) Prepare a minimum of 1000 aliquots of each DNA preparation in ultraclean microfuge tubes that are suitable for storage at -80 °C for extended periods. Each aliquot/tube will contain 20.0 µl of 500 ng/µl purified DNA. Additionally, the Contractor would be required to provide for each microbial isolate: 1) Glycerol stocks (10 x 1 ml) of cells obtained from the same liquid culture from which DNA was obtained/extracted. Supplied frozen at -80 °C or on dry ice. 2) Agarose plugs of cells (10 plugs per isolate) obtained from the same liquid culture from which DNA was obtained/extracted. Standard protocols for preparing agarose plugs will be provided by NIST. 3) A certificate of analysis for each DNA preparation demonstrating: a. A photograph of the petri dish from which a single (pure) colony was isolated to inoculate the liquid culture. b. DNA purity as measured by UV spectrophotometry absorbance. A260/A280 >1.8 c. Average DNA fragment size is >100 kilobases. As demonstrated by gel electrophoresis or Agilent Bioanalyzer or other molecular method(s). Concentration UV absorbance and Florescence Spectrometry (e.g. Qubit) The following information is being requested, if available: 1. An estimated cost per isolate, given the previously detailed constraints. To the extent that economies of scale exist, please include that information as it will inform this decision. The Contractor may include any relevant other cost-related information at its discretion. 2. Relevant documentation that demonstrates the Contractor's experience with manufacturing purified DNA from cultured bacterial isolates at this scale within the past five years. 3. Relevant documentation developing NIST Traceable Reference Materials (NTRMs) or producing NIST RMs within the past five years 4. Relevant documentation that demonstrates the Contractor's ability to produce said materials with documented long term stability. Long term in this context refers to 3-5 years or more from the date of manufacture under appropriate storage conditions, with respect to the criteria listed above. 5. Any other relevant information that is not listed above which the Government should consider in finalizing its market research. Responses are limited to a total of five (5) pages of letter size paper with single spacing. Responses must be received via email to erik.frycklund@nist.gov no later than March 25th, 2016, at 12:00 PM Eastern Time.
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