SOLICITATION NOTICE
B -- Use of Mouse Knockout Models to Determine the Functions of Insulin in Taste Bud Cells - Solicitation Document
- Notice Date
- 3/24/2016
- Notice Type
- Combined Synopsis/Solicitation
- NAICS
- 541711
— Research and Development in Biotechnology
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Institute on Drug Abuse, Station Support/Simplified Acquisitions, 31 Center Drive, Room 1B59, Bethesda, Maryland, 20892, United States
- ZIP Code
- 20892
- Solicitation Number
- HHS-NIH-NIDA-SSSA-2016-280
- Archive Date
- 4/13/2016
- Point of Contact
- Brian Lind, Phone: 3014021635
- E-Mail Address
-
lindbj@nida.nih.gov
(lindbj@nida.nih.gov)
- Small Business Set-Aside
- Total Small Business
- Description
- Solicitation Document INTRODUCTION: This is a solicitation for commercial items prepared in accordance with the format in FAR Part 12 as supplemented with additional information included in this notice. This announcement constitutes the only written solicitation; proposals are being requested and a separate solicitation will not be issued. The solicitation number is HHS-NIH-NIDA-SSSA-2016-280 and the solicitation is issued as a request for proposal (RFP). The Government intends to issue a firm fixed price purchase order for this requirement. ACQUISITION AUTHORITY: This acquisition is for a commercial service and is conducted under the authority of the Federal Acquisition Regulation (FAR) Part 13-Simplified Acquisition Procedures; FAR Subpart 13.5-Test Program for Certain Commercial Items; and FAR Part 12-Acquisition of Commercial Items, and is not expected to exceed the simplified acquisition threshold. The solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Circular 2005-87 dated March 7, 2016. NORTH AMERICAN INDUSTRY CLASSIFICATION SYSTEM (NAICS) CODE: The associated NAICS code is 541711 (Research and Development in Biotechnology) and the small business size standard is 500 employees. BACKGROUND: The National Institute on Aging (NIA) is conducting a project to determine the function of insulin in the taste buds cells of the tongue by specifically deleting insulin in the taste bud cells of the tongue of mice but allowing for normal expression of insulin in the islets of Langerhans in the pancreas. DESCRIPTION OF REQUIREMENT: The purpose of this requirement is to procure production services for three mouse models and produce a fourth mouse model which will help to determine the function of insulin in the taste buds cells of the tongue by specifically deleting insulin in the taste bud cells of the tongue of mice but allowing for normal expression of insulin in the islets of Langerhans in the pancreas. Project Requirements: Specifically, the Contractor shall provide the following services for mice that will be mated for up to three (3) months or until up to 25 tails have been genotyped: The contractor is required to make three transgenic mouse models and provide at least one breeding pair of homozygous mice for each of these mouse models to NIA. Contractor shall use sophisticated molecular biology tools and their expertise to determine how to do this exactly and will make the constructs to inject into the ES cell lines and then breed the mice to get at least one breeding pair of homozygous mice for each of the three mouse models. Mice of each model constitute a deliverable to the NIA. The contractor will then cross two of these mouse models to produce a fourth mouse model and provide NIA with at least one breeding pair of mice homozygous for the two genes in question. The four mouse models shall be generated as follows: 1. Mouse Model # 1 Mouse model in which the insulin 1 gene is absent in the entire animal i.e. a conventional knockout of the insulin 1 gene. 2. Mouse Model # 2 Mouse model in which the insulin 2 gene is absent in the entire animal i.e. a conventional knockout of the insulin 2 gene. 3. Mouse Model # 3 Mouse model in which the insulin 2 gene is floxed in the entire animal i.e. a mouse that will allow NIA to make a conditional knockout of the insulin 2 gene. 4. Mouse Model #4 Contractor shall breed mouse model # 1 with mouse model #3 to generate mice that are homozygous for both mutations. Contractor shall provide the NIA with at least one breeding pair of mice that are homozygous for both transgenes. Deliverables: Contractor shall provide monthly written reports at each major milestone for the testing procedures. Contractor shall provide a mapping of the gene for each mouse model. Contractor shall provide animal health certification reports that must be supplied with each shipment of mice to the NIA vivarium. These must include certification that mice are free of pinworm infection, as evidenced by Polymerase Chain Reaction (PCR) Assays. ANTICIPATED PERIOD OF PERFORMANCE: The anticipated period of performance is within eight (8) months after receipt of the purchase order. Period of performance will be defined upon award and the anticipated delivery is: NIH/National Institute on Aging Vivarium Biomedical Research Center (BRC) 251 Bayview Boulevard Suite 100 Baltimore, MD 21224 See the attached solicitation document for complete specifications and details as well as the technical and business proposal instructions.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NIDA-2/HHS-NIH-NIDA-SSSA-2016-280/listing.html)
- Place of Performance
- Address: NIH/National Institute on Aging Vivarium, Biomedical Research Center (BRC), 251 Bayview Boulevard, Suite 100, Baltimore, Maryland, 21224, United States
- Zip Code: 21224
- Zip Code: 21224
- Record
- SN04062270-W 20160326/160324235417-f0e2729f9476d0a8cfa4d096538b94b7 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
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