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FBO DAILY - FEDBIZOPPS ISSUE OF AUGUST 21, 2016 FBO #5385
SOURCES SOUGHT

B -- Immunosenescence-Associated Changes in Gene Expression and Chromatin Structure - Sources Sought 4241155

Notice Date

8/19/2016
 

Notice Type

Sources Sought
 

NAICS

541380 — Testing Laboratories
 

Contracting Office

Department of Health and Human Services, National Institutes of Health, National Institute on Drug Abuse, 6001 Executive Boulevard, Room 3155, MSC 9593, Bethesda, Maryland, 20892, United States
 

ZIP Code

20892
 

Solicitation Number

HHS-NIH-NIDA-SSSA-SS-543
 

Archive Date

9/13/2016
 

Point of Contact

Danielle R. Brown, Phone: 301 594 1928
 

E-Mail Address

danielle.brown2@nih.gov
(danielle.brown2@nih.gov)
 

Small Business Set-Aside

N/A
 

Description

Sources Sought document for HHS-NIH-NIDA-SSSA-SS-543 The proposed project will examine age-associated changes in gene expression and chromatin structure in primary human cell subsets isolated from peripheral blood. The goal for 2016/2017 is to carry out the analysis for 30 donors spanning the age-range from 20-80 years. Cytopheresis samples will be fractionated by a combination of magnetic bead purification and flow cytometry into 10 cell subsets. RNA isolated from each cell subset and total peripheral blood mononuclear cells (PBMC) will be sequenced to identify age-associated changes and develop algorithms to de-convolute data from PBMC. Chromatin immunoprecipitation (ChIP) and DNase I hypersensitivity analysis will be carried out using antibodies directed against specific histone modifications and ATAC protocol, respectively. For these studies we will use only PBMC samples and the most abundant cell subsets to start with. Purified RNA and DNA from ChIP samples will be provided to the chosen contractor for Hi-Seq analysis. ATAC samples will be converted into libraries at the NIA and provided to the facility for sequencing.
 

Web Link

FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NIDA-01/HHS-NIH-NIDA-SSSA-SS-543/listing.html)
 

Place of Performance

Address: 251 Bayview Blvd, Suite 100, Baltimore, Maryland, 21224, United States

Zip Code: 21224
 

Record

SN04233019-W 20160821/160819235455-98bdff89a696968f46950861e50a2696 (fbodaily.com)
 

Source

FedBizOpps Link to This Notice
(may not be valid after Archive Date)

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