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FBO DAILY - FEDBIZOPPS ISSUE OF SEPTEMBER 09, 2016 FBO #5404
SOLICITATION NOTICE

R -- Immunosenescence and Inflammation Associated with Aging - Pre-Solicitation Notice of Intent NONCOMPETITIVE

Notice Date

9/7/2016
 

Notice Type

Presolicitation
 

NAICS

541380 — Testing Laboratories
 

Contracting Office

Department of Health and Human Services, National Institutes of Health, National Institute on Drug Abuse, 6001 Executive Boulevard, Room 3155, MSC 9593, Bethesda, Maryland, 20892, United States
 

ZIP Code

20892
 

Solicitation Number

NIDA-NIH-SSSA-2016-PSOL-543
 

Archive Date

9/27/2016
 

Point of Contact

Danielle R. Brown, Phone: 301 480 2385, Rachelle Trice, Phone: 301 594 1928
 

E-Mail Address

danielle.brown2@nih.gov, rachelle.trice@nih.gov
(danielle.brown2@nih.gov, rachelle.trice@nih.gov)
 

Small Business Set-Aside

N/A
 

Description

Pre-Solicitation Notice of Intent Non-Competitive The proposed project will examine age-associated changes in gene expression and chromatin structure in primary human cell subsets isolated from peripheral blood. The goal for 2016/2017 is to carry out the analysis for 30 donors spanning the age-range from 20-80 years. Cytopheresis samples will be fractionated by a combination of magnetic bead purification and flow cytometry into 10 cell subsets. RNA isolated from each cell subset and total peripheral blood mononuclear cells (PBMC) will be sequenced to identify age-associated changes and develop algorithms to de-convulute data from PBMC. Chromatin immunoprecipitation (ChIP) and DNAse I hypersensitivity analysis will be carried out using antibodies directed against specific histone modifications and ATAC protocol, respectively. For these studies we will use only PBMC samples and the most abundant cell subsets to start with. Purified RNA and DNA from ChIP samples will be provided to the Johns Hopkins Deep Sequencing and Microarray Core for Hi-Seq analysis. ATAC samples will be converted into libraries at the NIA and provided to the facility for sequencing.
 

Web Link

FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NIDA-01/NIDA-NIH-SSSA-2016-PSOL-543/listing.html)
 

Place of Performance

Address: 251 Bayview Boulevard, Suite 100, Room 06b113, Baltimore, Maryland, 21224, United States

Zip Code: 21224
 

Record

SN04258988-W 20160909/160907234737-aa221a9a4010c924cb58badeeb300403 (fbodaily.com)
 

Source

FedBizOpps Link to This Notice
(may not be valid after Archive Date)

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