SOURCES SOUGHT
99 -- Single Cell RNA Sequencing of Basal Forebrain GABAergic Neurons
- Notice Date
- 8/25/2017
- Notice Type
- Sources Sought
- NAICS
- 325414
— Biological Product (except Diagnostic) Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Institute on Drug Abuse, 6001 Executive Boulevard, Room 4211, MSC 9559, Bethesda, Maryland, 20892-9559, United States
- ZIP Code
- 20892-9559
- Solicitation Number
- HHS-NIH-NIDA-SSSA-SS-17-620
- Archive Date
- 9/14/2017
- Point of Contact
- Danielle R. Brown, Phone: 301 480 2385
- E-Mail Address
-
danielle.brown2@nih.gov
(danielle.brown2@nih.gov)
- Small Business Set-Aside
- N/A
- Description
- Federal Business Opportunities (FBO) SOURCES SOUGHT NOTICE 1.Solicitation Number: HHS-NIH-NIDA-SSSA-SS-17-620 2.Title: Single Cell RNA Sequencing of Basal Forebrain GABAergic Neurons 3.NAICS Code: 325414 - Biological Product (Except Diagnostic) Manufacturing 4.Size Standard: 1,250 Employees 5.Description: This is a Sources Sought notice. This is NOT a solicitation for proposals, proposal abstracts, or quotations. The purpose of this notice is to obtain information regarding the availability and capability of all qualified sources to perform a potential requirement. This notice is issued to help determine the availability of qualified companies technically capable of meeting the Government requirement and to determine the method of acquisition. It is not to be construed as a commitment by the Government to issue a solicitation or ultimately award a contract. Responses will not be considered as proposals or quotes. No award will be made as a result of this notice. The Government will NOT be responsible for any costs incurred by the respondents to this notice. This notice is strictly for research and information purposes only. Background: Research in the Neural Circuits and Cognition Unit (NCCU) seeks to establish noncholinergic basal forebrain (BF) neurons as a key neural mechanism in decision making and top-down attention. The importance of this research is that it will provide the necessary foundation to understand how noncholinergic BF neurons contribute to conditions in which decision making and top-down attention are impaired, including aging, Alzheimer's disease, schizophrenia, and ADHD. A major component of our research program is to determine the neurochemical identity of BF bursting neurons. This is a critical step to provide mechanistic insights about this neuronal circuitry, which paves the way to study this system using other experimental approaches and can be extended to other species including humans. To achieve this goal, in this current project we seek to determine the RNA expression patterns of GABAergic neurons in the basal forebrain. Specifically, we use laser capture microdissection technique to harvest individual GFP-labeled GABAergic neurons from mouse brains. These samples of individual neurons (288 sample neurons) are then sequenced for RNA expression pattern. The reagents purchased in this request are used to convert the RNAs into cDNAs, amplify the cDNA amount, and add specific tags to label each single cell. These are the needed steps for the single cell RNA sequencing. Due to the very tiny amount of RNA present in each single cell sample, special processing kits from Takara are needed. Purpose and Objectives: The purpose of this acquisition is to purchase the necessary reagents to conduct single cell RNA sequencing experiments. This experiment will reveal previously unknown information about the genetic makeup of GABAergic neurons in the basal forebrain, a brain region implicated in age-related cognitive decline and various forms of dementia. This is a continuation of a small scale pilot experiment, in which the experimental conditions have been tested and validated. Purchase Description: SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing; from Takara Bio USA, part number 634891/634892 Low Input Library Prep Kit; from Takara Bio USA, part number 634900 Salient characteristics These specialized reagents need to meet two particular constraints: (1) must be able to process tiny amount of RNAs present in single cells (10 pg of RNA), while maintaining high reproducibility, even gene body coverage, and excellent representation of GC-rich transcripts; (2) must be able to work with existing high-throughput sequencing equipment (Illumina®) currently in the Gene Expression and Genomics Unit, NIA IRP. After consulting with Dr. Kevin Becker, head of the Gene Expression and Genomics Unit, NIA IRP, it was determined that the two TAKARA products (SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing; Low Input Library Prep Kit) are what we needed. (3) We have carried out small scale pilot experiments for 24 single cells to make sure that the entire experiment works as expected. Based on the successful pilot experiment, we are now scaling up to 288 single cells. Quantity: •SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing: 288 reactions (part number 634891 has 96 reactions; part number 634892 has 192 reactions) •Low Input Library Prep Kit HT x 3 sets (96 reactions each, total of 288 reactions) Delivery Date: Within 30 days after receipt of this order (ARO). Capability statement /information sought. Please provide a capability statement, this includes: •Provide evidence the respondent has performed services. •Provide evidence of similar previous contracts of research of the same magnitude. •Information regarding (a) staff expertise, including their availability, experience, and formal and other training; (b) current in-house capability and capacity to perform the work; (c) prior completed project of similar nature; (d) corporate experience and management capability; and (e) examples of prior completed Government contracts, references, and other related information. Please provide your DUNS number, organization name, address point of contact, and size and type of business (e.g., 8(a), HubZone, etc.,) pursuant to the applicable NAICS code and any other information that may be helpful. One (1) copy of the response is required and must be in Microsoft Word or Adobe PDF format using 11-point or 12-point font, 8-1/2" x 11" paper size, with 1" top, bottom, left and right margins, and with single or double spacing. The information submitted must be must be in and outline format that addresses each of the elements of the project requirement and in the capability statement /information sought paragraphs stated herein. The response is limited to one to two (1-2) page limit. The 1-2 page(s) do not include the cover page, executive summary, or references, if requested. All responses to this notice must be submitted electronically to the Contract Specialist and Contracting Officer. Facsimile responses are NOT accepted. The response must be submitted to Danielle R. Brown, Contract Specialist, at Danielle.brown2@nih.gov. The response must be received on or before Wednesday, August 30, 2017 at 12:00PM, Eastern Standard Time. "Disclaimer and Important Notes: This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization's qualifications to perform the work. Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After a review of the responses received, a pre-solicitation synopsis and solicitation may be published in Federal Business Opportunities. However, responses to this notice will not be considered adequate responses to a solicitation. Confidentiality: No proprietary, classified, confidential, or sensitive information should be included in your response. The Government reserves the right to use any non-proprietary technical information in any resultant solicitation(s)." PURCHASE DESCRIPTION 1.Statement of Need and Purpose: The purpose of this acquisition is to purchase the necessary reagents to conduct single cell RNA sequencing experiments. This experiment will reveal previously unknown information about the genetic makeup of GABAergic neurons in the basal forebrain, a brain region implicated in age-related cognitive decline and various forms of dementia. This is a continuation of a small scale pilot experiment, in which the experimental conditions have been tested and validated. 2.Background Information and Objective: Research in the Neural Circuits and Cognition Unit (NCCU) seeks to establish noncholinergic basal forebrain (BF) neurons as a key neural mechanism in decision making and top-down attention. The importance of this research is that it will provide the necessary foundation to understand how noncholinergic BF neurons contribute to conditions in which decision making and top-down attention are impaired, including aging, Alzheimer's disease, schizophrenia, and ADHD. A major component of our research program is to determine the neurochemical identity of BF bursting neurons. This is a critical step to provide mechanistic insights about this neuronal circuitry, which paves the way to study this system using other experimental approaches and can be extended to other species including humans. To achieve this goal, in this current project we seek to determine the RNA expression patterns of GABAergic neurons in the basal forebrain. Specifically, we use laser capture microdissection technique to harvest individual GFP-labeled GABAergic neurons from mouse brains. These samples of individual neurons (288 sample neurons) are then sequenced for RNA expression pattern. The reagents purchased in this request are used to convert the RNAs into cDNAs, amplify the cDNA amount, and add specific tags to label each single cell. These are the needed steps for the single cell RNA sequencing. Due to the very tiny amount of RNA present in each single cell sample, special processing kits from Takara are needed. 3.Generic Name of Product: The generic name for "SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing" is single cell RNA to cDNA conversion kit The generic name for "Low Input Library Prep Kit" is single cell cDNA library preparation kit 4.Purchase Description: SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing; from Takara Bio USA, part number 634891/634892 Low Input Library Prep Kit; from Takara Bio USA, part number 634900 5.Salient characteristics These specialized reagents need to meet two particular constraints: (1) must be able to process tiny amount of RNAs present in single cells (10 pg of RNA), while maintaining high reproducibility, even gene body coverage, and excellent representation of GC-rich transcripts; (2) must be able to work with existing high-throughput sequencing equipment (Illumina®) currently in the Gene Expression and Genomics Unit, NIA IRP. After consulting with Dr. Kevin Becker, head of the Gene Expression and Genomics Unit, NIA IRP, it was determined that the two TAKARA products (SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing; Low Input Library Prep Kit) are what we needed. (3) We have carried out small scale pilot experiments for 24 single cells to make sure that the entire experiment works as expected. Based on the successful pilot experiment, we are now scaling up to 288 single cells. 6.Quantity: SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing: 288 reactions (part number 634891 has 96 reactions; part number 634892 has 192 reactions) Low Input Library Prep Kit HT x 3 sets (96 reactions each, total of 288 reactions) 7.Delivery Date: Within 30 days after receipt of this order
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NIDA-01/HHS-NIH-NIDA-SSSA-SS-17-620/listing.html)
- Place of Performance
- Address: 251 Bayview Boulevard, Baltimore, Maryland, 21224, United States
- Zip Code: 21224
- Zip Code: 21224
- Record
- SN04646840-W 20170827/170825231222-a5dacefd32290205f7c2301323fb56b5 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
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