SOLICITATION NOTICE
99 -- Single Cell RNA Sequencing of Basal Forebrain GABAergic Neurons
- Notice Date
- 9/18/2017
- Notice Type
- Presolicitation
- NAICS
- 325414
— Biological Product (except Diagnostic) Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Institute on Drug Abuse, 6001 Executive Boulevard, Room 4211, MSC 9559, Bethesda, Maryland, 20892-9559, United States
- ZIP Code
- 20892-9559
- Solicitation Number
- HHS-NIH-NIDA-SSSA-NOI-17-620
- Archive Date
- 10/10/2017
- Point of Contact
- Danielle R. Brown, Phone: 301 480 2385
- E-Mail Address
-
danielle.brown2@nih.gov
(danielle.brown2@nih.gov)
- Small Business Set-Aside
- N/A
- Description
- PRE-SOLICITATION NOTICE OF INTENT NON-COMPETITIVE Solicitation: HHS-NIH-NIDA-SSSA-NOI-17-620 Title: Single Cell RNA Sequencing of Basal Forebrain GABAergic Neurons INTRODUCTION PURSUANT TO FAR Subpart 5.2-Synopses of Proposed Contract Actions, THIS IS A PRE-SOLICITATION NOTICE OF A PROPOSED CONTRACT TO ACTION. THIS IS A PRE-SOLICITATION NON-COMPETITIVE NOTICE OF INTENT TO AWARD A CONTRACT OR PURCHASE ORDER WITHOUT PROVIDING FOR FULL OR OPEN COMPETITION (INCLUDING BRAND-NAME). The National Institute on Drug Abuse (NIDA), Station Support Contracts and Simplified Acquisitions Branch (SS/SA) on behalf of the National Institute on Aging (NIA) intends to negotiate and award a contract for the Single Cell RNA Sequencing Reagents with Takara Bio USA, Inc., 1290 Terra Bella Avenue, Mountain View, CA, 94043-1837. NORTH AMERICAN INDUSTRY CLASSIFICATION SYSTEM (NAICS) CODE The intended procurement is classified under NAICS code 325414 - Biological Product (except Diagnostic) Manufacturing with a Size Standard of 1,250 Employees. REGULATORY AUTHORITY The resultant contract will include all applicable provisions and clauses of the Federal acquisition Requlation (FAR) in effect through the Federal Acquisition Circular (FAC) 05-95, January 19, 2017. STATUTORY AUTHORITY This acquisition is conducted as non-competitive under the authority of 41 U.S.C. 253(c) under provisions of the statutory authority of FAR Subpart 6.302-1 - Only one response source and no other supplies or services will satisfy agency requirements. DESCRIPTION OF REQUIREMENT Project Description Research in the Neural Circuits and Cognition Unit (NCCU) seeks to establish noncholinergic basal forebrain (BF) neurons as a key neural mechanism in decision making and top-down attention. The importance of this research is that it will provide the necessary foundation to understand how noncholinergic BF neurons contribute to conditions in which decision making and top-down attention are impaired, including aging, Alzheimer's disease, schizophrenia, and ADHD. A major component of our research program is to determine the neurochemical identity of BF bursting neurons. This is a critical step to provide mechanistic insights about this neuronal circuitry, which paves the way to study this system using other experimental approaches and can be extended to other species including humans. To achieve this goal, in this current project we seek to determine the RNA expression patterns of GABAergic neurons in the basal forebrain. Specifically, we use laser capture microdissection technique to harvest individual GFP-labeled GABAergic neurons from mouse brains. These samples of individual neurons (288 sample neurons) are then sequenced for RNA expression pattern. The reagents purchased in this request are used to convert the RNAs into cDNAs, amplify the cDNA amount, and add specific tags to label each single cell. These are the needed steps for the single cell RNA sequencing. Due to the very tiny amount of RNA present in each single cell sample, special processing kits from Takara are needed. Purpose and Objectives The purpose of this acquisition is to purchase the necessary reagents to conduct single cell RNA sequencing experiments. This experiment will reveal previously unknown information about the genetic makeup of GABAergic neurons in the basal forebrain, a brain region implicated in age-related cognitive decline and various forms of dementia. This is a continuation of a small scale pilot experiment, in which the experimental conditions have been tested and validated. Delivery Schedule Within 30 days after receipt of order. CLOSING STATEMENT This synopsis is not a request for competitive proposals. However, interested parties may identify their interest and capability to respond to this notice. Responses to this solicitation must include clear and convincing evidence of the offeror's capability of fulfilling the requirement as it relates to the technical evaluation criteria. The price proposal must include the labor categories, an estimate of the number of hours required for each labor category, fully loaded fixed hourly rate or each labor category, breakdown and rationale for other direct costs or materials, and the total amount. Salient characteristics: SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing; from Takara Bio USA, part number 634891/634892 Low Input Library Prep Kit; from Takara Bio USA, part number 634900 These specialized reagents need to meet two particular constraints: (1) must be able to process tiny amount of RNAs present in single cells (10 pg of RNA), while maintaining high reproducibility, even gene body coverage, and excellent representation of GC-rich transcripts; (2) must be able to work with existing high-throughput sequencing equipment (Illumina®) currently in the Gene Expression and Genomics Unit, NIA IRP. After consulting with Dr. Kevin Becker, head of the Gene Expression and Genomics Unit, NIA IRP, it was determined that the two TAKARA products (SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing; Low Input Library Prep Kit) are what we needed. (3) We have carried out small scale pilot experiments for 24 single cells to make sure that the entire experiment works as expected. Based on the successful pilot experiment, we are now scaling up to 288 single cells. Quantity: SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing: 288 reactions (part number 634891 has 96 reactions; part number 634892 has 192 reactions) Low Input Library Prep Kit HT x 3 sets (96 reactions each, total of 288 reactions) See attached Purchase Description (PD) for further information. In addition the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size must be included in the response. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov." A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. The information received will normally be considered solely for the purposes of determining whether to proceed on a non-competitive basis or to conduct a competitive procurement. All responses must be received by Monday, September 25, 2017 at 10AM, Eastern Standard Time, and reference number HHS-NIH-NIDA-SSSA-NOI-17-620. Responses may be submitted electronically to Danielle R. Brown, Contract Specialist, at Danielle.brown2@nih.gov. Fax responses will not be accepted. Please contact Danielle R. Brown, Contract Specialist, at Danielle.brown2@nih.gov or (301) 594-1928 with any questions. PURCHASE DESCRIPTION 1.Statement of Need and Purpose: The purpose of this acquisition is to purchase the necessary reagents to conduct single cell RNA sequencing experiments. This experiment will reveal previously unknown information about the genetic makeup of GABAergic neurons in the basal forebrain, a brain region implicated in age-related cognitive decline and various forms of dementia. This is a continuation of a small scale pilot experiment, in which the experimental conditions have been tested and validated. 2.Background Information and Objective: Research in the Neural Circuits and Cognition Unit (NCCU) seeks to establish noncholinergic basal forebrain (BF) neurons as a key neural mechanism in decision making and top-down attention. The importance of this research is that it will provide the necessary foundation to understand how noncholinergic BF neurons contribute to conditions in which decision making and top-down attention are impaired, including aging, Alzheimer's disease, schizophrenia, and ADHD. A major component of our research program is to determine the neurochemical identity of BF bursting neurons. This is a critical step to provide mechanistic insights about this neuronal circuitry, which paves the way to study this system using other experimental approaches and can be extended to other species including humans. To achieve this goal, in this current project we seek to determine the RNA expression patterns of GABAergic neurons in the basal forebrain. Specifically, we use laser capture microdissection technique to harvest individual GFP-labeled GABAergic neurons from mouse brains. These samples of individual neurons (288 sample neurons) are then sequenced for RNA expression pattern. The reagents purchased in this request are used to convert the RNAs into cDNAs, amplify the cDNA amount, and add specific tags to label each single cell. These are the needed steps for the single cell RNA sequencing. Due to the very tiny amount of RNA present in each single cell sample, special processing kits from Takara are needed. 3.Generic Name of Product: The generic name for "SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing" is single cell RNA to cDNA conversion kit The generic name for "Low Input Library Prep Kit" is single cell cDNA library preparation kit 4.Purchase Description: SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing; from Takara Bio USA, part number 634891/634892 Low Input Library Prep Kit; from Takara Bio USA, part number 634900 5.Salient characteristics These specialized reagents need to meet two particular constraints: (1) must be able to process tiny amount of RNAs present in single cells (10 pg of RNA), while maintaining high reproducibility, even gene body coverage, and excellent representation of GC-rich transcripts; (2) must be able to work with existing high-throughput sequencing equipment (Illumina®) currently in the Gene Expression and Genomics Unit, NIA IRP. After consulting with Dr. Kevin Becker, head of the Gene Expression and Genomics Unit, NIA IRP, it was determined that the two TAKARA products (SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing; Low Input Library Prep Kit) are what we needed. (3) We have carried out small scale pilot experiments for 24 single cells to make sure that the entire experiment works as expected. Based on the successful pilot experiment, we are now scaling up to 288 single cells. 6.Quantity: SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing: 288 reactions (part number 634891 has 96 reactions; part number 634892 has 192 reactions) Low Input Library Prep Kit HT x 3 sets (96 reactions each, total of 288 reactions) 7.Delivery Date: Within 30 days after receipt of this order
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NIDA-01/HHS-NIH-NIDA-SSSA-NOI-17-620/listing.html)
- Place of Performance
- Address: 251 Bayview Boulevard, Baltimore, Maryland, 21224, United States
- Zip Code: 21224
- Zip Code: 21224
- Record
- SN04683281-W 20170920/170918232433-d613e1a8ce4d4f40f9a6cbf036103874 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
| FSG Index | This Issue's Index | Today's FBO Daily Index Page |