SOLICITATION NOTICE
66 -- Procurement of All-In-One Fluorescence Microscope
- Notice Date
- 9/18/2017
- Notice Type
- Presolicitation
- NAICS
- 333314
— Optical Instrument and Lens Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Institute on Drug Abuse, 6001 Executive Boulevard, Room 4211, MSC 9559, Bethesda, Maryland, 20892-9559, United States
- ZIP Code
- 20892-9559
- Solicitation Number
- HHS-NIH-NIDA-SSSA-NOI-17-764
- Archive Date
- 10/10/2017
- Point of Contact
- Danielle R. Brown, Phone: 301 480 2385
- E-Mail Address
-
danielle.brown2@nih.gov
(danielle.brown2@nih.gov)
- Small Business Set-Aside
- N/A
- Description
- PRE-SOLICITATION NOTICE OF INTENT NON-COMPETITIVE Solicitation: HHS-NIH-NIDA-SSSA-NOI-17-764 Title: Procurement of All-In-One Fluorescence Microscope INTRODUCTION PURSUANT TO FAR Subpart 5.2-Synopses of Proposed Contract Actions, THIS IS A PRE-SOLICITATION NOTICE OF A PROPOSED CONTRACT TO ACTION. THIS IS A PRE-SOLICITATION NON-COMPETITIVE NOTICE OF INTENT TO AWARD A CONTRACT OR PURCHASE ORDER WITHOUT PROVIDING FOR FULL OR OPEN COMPETITION (INCLUDING BRAND-NAME). The National Institute on Drug Abuse (NIDA), Station Support Contracts and Simplified Acquisitions Branch (SS/SA) on behalf of the National Institute on Aging (NIA) intends to negotiate and award a contract for the All-In-One Fluorescence Microscope with Keyence Corporation of America, 669 River Drive, Suite 403, Elmwood Park, NJ 07407. NORTH AMERICAN INDUSTRY CLASSIFICATION SYSTEM (NAICS) CODE The intended procurement is classified under NAICS code 333314 - Optical Instrument and Lens Manufacturing with a Size Standard of 500 Employees. REGULATORY AUTHORITY The resultant contract will include all applicable provisions and clauses of the Federal acquisition Requlation (FAR) in effect through the Federal Acquisition Circular (FAC) 05-95, January 19, 2017. STATUTORY AUTHORITY This acquisition is conducted as non-competitive under the authority of 41 U.S.C. 253(c) under provisions of the statutory authority of FAR Subpart 6.302-1 - Only one response source and no other supplies or services will satisfy agency requirements. DESCRIPTION OF REQUIREMENT Project Description The All-in-one microscope will allow complete imaging of tissue microarrays mounted on slides, multi-well experiments assessing the role of transcription factors in cell differentiation and aging rates of human iPS cells. The instrument will allow live cell imaging as well. The potential NIA applications of this microscope include, but are not limited to: •Ongoing studies of senescence (Gorospe lab, LGG) and aging in mice (SLAM, de Cabo lab, TGB) which can be facilitated by the user-friendly, high throughput, semi-automated imaging of tissue microarrays. •Single cell DNA damage assays (Comet assays) can be more efficiently imaged (Beerman lab, TGB). •Mitochondrial volume reconstructions can be rendered for whole organisms, tissue culture or isolated mitochondria, complementing the use of Seahorse system for measuring mitochondrial ROS and ATP generation.(Tyler Demarest, Bohr lab, LMG). For example, cultures analyzed in Seahorse plates can be re-imaged with the Keyence microscope system. •Wound invasion assays of NF-kB reporter expressing fibroblasts can be assessed on this setup as well. (Erik Martin, Sung lab, LMBI) Purpose and Objectives The purpose of this acquisition is to procure a small stand-alone microscope with its own enclosed dark room with modular slide holders compatible with tissue culture flasks and plates, multi-well plates, coverslips and slides. The microscope must have bright field, fluorescence, and phase-contrast imaging with the capability for image stitching, Z-stack image capturing, and coordinate-specific condition setting. Delivery Schedule Within 30 days week after receipt of order (ARO). CLOSING STATEMENT This synopsis is not a request for competitive proposals. However, interested parties may identify their interest and capability to respond to this notice. Responses to this solicitation must include clear and convincing evidence of the offeror's capability of fulfilling the requirement as it relates to the technical evaluation criteria. The price proposal must include the labor categories, an estimate of the number of hours required for each labor category, fully loaded fixed hourly rate or each labor category, breakdown and rationale for other direct costs or materials, and the total amount. Salient characteristics: See attached Purchase Description for more details. Quantity: One (1) In addition the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size must be included in the response. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov." A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. The information received will normally be considered solely for the purposes of determining whether to proceed on a non-competitive basis or to conduct a competitive procurement. All responses must be received by Monday, September 25, 2017 at 10AM, Eastern Standard Time, and reference number HHS-NIH-NIDA-SSSA-NOI-17-764. Responses may be submitted electronically to Danielle R. Brown, Contract Specialist, at Danielle.brown2@nih.gov. Fax responses will not be accepted. Extensions to the deadline will not be granted. Please contact Danielle R. Brown, Contract Specialist, at Danielle.brown2@nih.gov or (301) 594-1928 with any questions. PURCHASE DESCRIPTION 1.Statement of Need and Purpose: The purpose of this acquisition is to procure a small stand-alone microscope with its own enclosed dark room with modular slide holders compatible with tissue culture flasks and plates, multi-well plates, coverslips and slides. The microscope must have bright field, fluorescence, and phase-contrast imaging with the capability for image stitching, Z-stack image capturing, and coordinate-specific condition setting. 2.Background Information and Objective: The All-in-one microscope will allow complete imaging of tissue microarrays mounted on slides, multi-well experiments assessing the role of transcription factors in cell differentiation and aging rates of human iPS cells. The instrument will allow live cell imaging as well. The potential NIA applications of this microscope include, but are not limited to: •Ongoing studies of senescence (Gorospe lab, LGG) and aging in mice (SLAM, de Cabo lab, TGB) which can be facilitated by the user-friendly, high throughput, semi-automated imaging of tissue microarrays. •Single cell DNA damage assays (Comet assays) can be more efficiently imaged (Beerman lab, TGB). •Mitochondrial volume reconstructions can be rendered for whole organisms, tissue culture or isolated mitochondria, complementing the use of Seahorse system for measuring mitochondrial ROS and ATP generation.(Tyler Demarest, Bohr lab, LMG). For example, cultures analyzed in Seahorse plates can be re-imaged with the Keyence microscope system. •Wound invasion assays of NF-kB reporter expressing fibroblasts can be assessed on this setup as well. (Erik Martin, Sung lab, LMBI) 3.Generic Name of Product: All-In-One fluorescence microscope 4.Purchase Description: 1BZ-X710 BZX Fluorescence Microscope 2BZ-X700E BZX Fluorescence Microscope 3972318 Win10 PC for BZ-X Dell T3420 4972072B BZX Accessory 23'' Monitor 5 BZ TRAININGBZ Training 6 BZ-H3XDBZX Software 7 BZ-H3XTBZX Software BZX Time Lapse 8 BZ-H3AEBZX Software BZX Analyzer Software 9 OP-87762BZX Accessory BZX DAPI Filter 10 OP-87763BZX Accessory BZX GFP Filter 11 OP-87765BZX Accessory BZX Texas Red Filter 12 OP-87766BZX Accessory BZX Cy5 Filter 13 972081CFI PLAN FLUOR DL 4X LENS 14 971936CFI PLAN FLUOR DL 10X LENS 15 971962Nikon Lens (20x ELWD Phase) for BZ S PI FI ELWD ADM 20xC 16 971963Nikon Lens (40x ELWD Phase) for BZ S PL FL ELWD ADM 40xC 17 972082INUG2-KIW STAGE TOP INCUBATOR 5.Salient characteristics: Microscope unitBasic optical systemInverted fluorescence phase contrast microscope Objective lensesNikon CFI 60 Series infinite optical system Observation modesBright field, Fluorescence (wide-field/sectioning), Phase contrast (PhL, Ph1, Ph2), Oblique illumination Objective lens switchingSix-mount electronic revolver Image-formation optical systemFixed image-forming lens, electronic LC filter insertion / removal unit Electronic XY stage100×64 mm stroke, 1 um pitch min. Electronic Z stage8 mm stroke, 0.1 um pitch min. Electronic filter turretUp to four filters can be mounted. Automatic position recognition and automatic excitation shutdown during filter replacement Fluorescent incident illuminationOptical sectioning system Fluorescence dimming mechanismElectronic dimming (0.3% 5% 10% 20% 40% 100%) Transmitted illumination optical systemOperating distance : 30 mm, Pop-up mechanism (with automatic lamp off function) Transmitted illumination mechanismElectronic switching, Electronic bright-field aperture (0% 20% 40% 60% 80% 100%) / Phase-contrast slit (Ph1 / Ph2) Transmitted light sourceLED 3.7W, 50,000 hours (average) Fluorescent incident light sourceMetal halide lamp 80W, 2,000 hours (average) Specimen enclosureThe stage is fully contained in a built-in darkroom. Camera unitImage pick-up element2 / 3 inch, 2.83 million pixel monochrome CCD (colourised with LC filter) CCD cooling mechanismPeltier cooling : 5°C 41°F (at ambient temperature of -25°C -13°F) Output signal, gradationsMonochrome 14bit / 8bit, 8 bit for RGB each Frame rate15 fps for monochrome recording (up to 95 fps with binning), 8.5 fps for color recording BinningOn-chip binning (2×2、3×3、4×4、8×8、12×12) Number of pixels in recorded image4,080 × 3,060 max (12.5 million pixels, high-quality interpolation) Video capture8-bit monochrome: 15 fps for 1,280 x 960 With binning: 28 fps for 960 x 720, 40 fps for 640 x 480, 50 fps for 480 x 360, 75 fps for 240 x 180, 95 fps for 160 x 120 Color: 8.5 fps for 1,280 x 960 Electronic shutterAuto : 1 / 7500 to 60 sec. (77 increments) Auto Exposure methodArbitrary area, average / peak Gain0dB、+6dB、+12dB、+18dB White balanceMANUAL, PUSH-SET Black balance Observation softwareMulti-color image capturing, Auto focus, Quick full-focusing, Scale display, Electronic revolver control, Electronic stage control ControllerApplicable OSWindows7 Professional 64bit SP1*1 PC interfaceUSB2.0 Ambient temperature+15 to +35°C 59 to 95°F Relative humidity35 to 80% RH (No condensation) DimensionsHead : 517 (H) × 340 (W) × 496 (D) mm*2 / Controller : 227.5 (H) × 125 (W) × 403 (D) mm WeightHead : 33 kg / Controller : 4.8 kg Power voltage100 to 240 VAC ±10% 50 / 60 Hz Power consumption250VA Overvoltage categoryII Pollution degree2 Software (optional)Sectioning ModuleOptical sectioning image mode Advanced Observation ModuleTypeNavigation, Image stitching, Z-stack image capturing, Coordinate-specific condition setting Navigation ModuleNavigation Image stitching ModuleImage stitching, Z-stack image capturing, Coordinate-specific condition setting Time-lapse ModuleTime-lapse imaging, Video capturing Advanced Analysis SoftwareImage stitching, Haze reduction, Full focus Measurement ModuleXY measurement, Area measurement, Brightness measurement (line profile, histogram) 3D ModuleReal-time 3D analysis, XYZ slicing, Maximum projection Hybrid Cell Count ModuleCell count (phase contrast, bright field, fluorescence images), Mask cell count Macro Cell Count ModuleMacro Cell Count (batch analysis of multiple images) 6.Quantity: One (1) 7.Delivery Date: Within 30 Days after receipt of order (ARO)
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NIDA-01/HHS-NIH-NIDA-SSSA-NOI-17-764/listing.html)
- Place of Performance
- Address: 251 Bayview Boulevard, Baltimore, Maryland, 21224, United States
- Zip Code: 21224
- Zip Code: 21224
- Record
- SN04683302-W 20170920/170918232443-da188fab86c8e712585eea143f6e091f (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
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