SOURCES SOUGHT
65 -- Reagent mulitiplex chip kits for existing 10X Genomic Chromium Single Cell Controller
- Notice Date
- 8/3/2018
- Notice Type
- Sources Sought
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Institute on Drug Abuse, 6001 Executive Boulevard, Room 4211, MSC 9559, Bethesda, Maryland, 20892-9559, United States
- ZIP Code
- 20892-9559
- Solicitation Number
- NIHDA201800414
- Archive Date
- 8/25/2018
- Point of Contact
- Hunter A Tjugum, Phone: 3018275304
- E-Mail Address
-
hunter.tjugum@nih.gov
(hunter.tjugum@nih.gov)
- Small Business Set-Aside
- N/A
- Description
- INTRODUCTION This is a Small Business Sources Sought (SBSS) notice. This is NOT a solicitation for proposals, proposal abstracts, or quotations. The purpose of this notice is to obtain information regarding: (1) the availability and capability of qualified small business sources; (2) whether they are small businesses; HUBZone small businesses; service-disabled, veteran-owned small businesses; 8(a) small businesses; veteran-owned small businesses; woman-owned small businesses; or small disadvantaged businesses; and (3) their size classification relative to the North American Industry Classification System (NAICS) code for the proposed acquisition. Your responses to the information requested will assist the Government in determining the appropriate acquisition method, including whether a set-aside is possible. An organization that is not considered a small business under the applicable NAICS code should not submit a response to this notice. This notice is issued to help determine the availability and type of qualified Small Business companies technically capable of meeting this potential government requirement and to determine the method of acquisition. It is not to be construed as a commitment by the Government to issue a solicitation or ultimately award a contract. Responses will not be considered as proposals or quotes. No award will be made as a result of this notice. The Government will NOT be responsible for any costs incurred by the respondents to this notice. The notice is strictly for research and information purposes only. The information requested will assist the Government in determining the appropriate acquisition method including small business socio-economic set-aside possibilities and to determine the availability of qualified Small Business companies technically capable of meeting the Government's potential requirement. All Small Business companies with the capability and availability to perform the requirement under the applicable NAICS code are invited to submit a response to this notice. NORTH AMERICAN CLASSIFICATION SYSTEM (NAICS) CODE The NAICS code applicable to this requirement is 334516 Analytical Laboratory Instrument Manufacturing with size standard 1000 employees. BACKGROUND The National Center for Advancing Translational Sciences (NCATS) at the National Institutes of Health (NIH) was officially established in fiscal year 2012 to transform the translational science process so that new treatments and cures for disease can be delivered to patients faster. NCATS, one of 27 Institutes and Centers (ICs) at NIH, strives to develop innovations to reduce, remove or bypass costly and time-consuming bottlenecks in the translational research pipeline in an effort to speed the delivery of new drugs, diagnostics and medical devices to patients. Through collaborations with industry, academia, patient advocacy and other nonprofit groups and in cooperation with other NIH ICs and government agencies, NCATS provides: Pre-clinical drug development expertise and access to resources; assistance generating data needed for regulatory approval; and a variety of mechanisms to streamline partnerships and collaborations. NCATS collaborates with investigators to study and develop compounds and molecules to further translational research. NCATS currently uses the 10X Genomics Chromium Single Cell Controller system, which is existing Government-owned equipment, manufactured bv 10X Genomics, located at 7068 Koll Center Parkway, Suite 401; Pleasanton, California 94566. The 10X Genomics Chromium Single Cell Controller system is in need of Reagent mulitiplex chip kits and are required for the consistency and continuity of ongoing stem cell research protocols and projects. Purpose and Objectives This Small Business Sources Sought notice seeks to determine eligible sources capable of achieving the essential features of this potential requirement. The purpose of this potential requirement is to obtain reagent mulitiplex chip kits to be compaitlbe with the existing Chromium Single Cell Controller, manufactured by 10x Genomic, as follows: 1. Chromium TM Single Cell Chip C Kit, 48 rxns (Product code: 1000022), quantity two (2); 2. Chromium TM Single Cell Chip D Kit, 48 rxns (Product code: 1000042), quantity two (2); 3. Chromium TM i7 Multiplex Kit, 96 rxns (Product code: 120262), quantity one (1); 4. Chromium TM Single Cell DNA accessory Kit (Product code: 1000058) quantity one (1); 5. Chromium TM Single Cell Bead Kit, 16 rxns (Prodcut code: 1000056), quantity five (5); 6. Chromium TM Single Cell DNA Library & Gel Bead Kit, 16 rxns (Prodcut code: 1000040), quantity five (5). The subject potential supply is required to perform single cell copy number profiling analysis by SCTL for the consistency and continuity of ongoing stem cell research protocols and projects. The reagent kits provide high throughput reagent delivery using the chip kits, which are microfluidic chips. The 8-sample Chip C cartridge is loaded with the gel beads, a cell suspension, cell beads and functionalized gel beads and an oil-surfactant solution. Reagents and cells are combined at a microfluidic junction where gel bead-containing emulsions (GEMs) are generated. GEMs are created in such a way that single cells are partitioned and >90% of reactions contain a unique cell-linked molecular barcode and samples a pool of ~750,000 10X barcodes to separately index each cell's genome. The 10X barcoded GEM mixture then transfers to Chip D cartridge for further enzymatic reactions to amplify DNA from individual cells. The simple workflow creates up to 100,000 cells per channel in a 7-minute instrument run time. The Chromium multiplex kit reagents are added in the amplification step of library construction for the product generated by the 10X Genomics Chromium Single Cell Controller and Chromium reagent kits. Ongoing projects on defining stem cell differentiation process in heterogeneous population require the examination of copy number variation profiling at single cell level. Genomic stability is critical for the cinical use of human induce pluripotent stem cells. Recent publication suggested dynamic nature of genomic abnormalities in pluripotent stem cells, therefore frequent genomic monitoring is essential for SCTL lab to develop reliable differentiation protocol for clinical grade stem cell derived cells. The Chromium reagent kits, chip kits, and multiplex kits are designed specifically for use with a current equipment "10X Genomics Chromium Single Cell Controller" based on the droplet-based reagent delivery method that uses a microfluidic chip to provide high-throughput single cell preparation and 65% cell capture efficiency. Project Requirements Specifically, the following salient features are deemed essential that this potential requirement will achieve: 1. Chromium TM Chromium TM Single Cell DNA Library & Gel Bead Kit, 16 rxns and Chromium TM Single Cell Bead Kit, 16 rxns: •Leverages Linked-Read data to reconstruct a diploid genome without the need for a reference •Routinely resolves the genome into extremely large haplotype phase blocks (i.e. >10 Mb) •Produces up to 1,000,000 individual nanoliter-scale reactions leveraging ~4,000,000 unique 16 bp barcodes •Generates Linked-Read molecules from >100 kb input molecules •Enables long-range phasing for whole genome, whole exome, and targeted resequencing applications to detect: o Haplotypes, which can be directly observed without the need for informatics inference oCompound heterozygosity oBalanced and unbalanced structural variant detection •Rescues short sequencing reads that reside in previously unmappable regions of the genome (frequently ~10% of all reads) oProvides stand-alone, turn-key analysis pipelines and visualization software fo novice and expert users alike for: •Sequence read demultiplexing •Barcode-aware alignment 2.Chromium TM Single Cell Chip C Kit, 48 rxns: •6 chips, 48 reactions •Loaded with the Cell Beads, and cell suspension to generate cell beads partition for following reaction. Cell beads will lyse the cell and remove all nuclear proteins while the genomic DNA remains trapped in cell beads. The reaction will proceed using chip D to generate generate cell beads and gel beads (CBGB) •Compatible with 10X Genomics Chromium Single Cell Controller 3.Chromium TM Single Cell Chip D Kit, 48 rxns: •6 chips, 48 reactions •Seqeuncital reaction from Chip C kit, cell beads with aingle cell genomic DNA from Chromium TM Single Cell Chip C Kit will load to Chip D to generate cell beads and gel beads (CBGB) to performe copy number variation profiling. •Compatible with 10X Genomics Chromium Single Cell Controller 4.Chromium TM i7 Multiplex Kit, 96 rxns •Contains ready-to-use balanced oligonucleotide mix in each well •Compatible with 10X Genomics Chromium Single Cell Controller •Compatible with Illumina HiSeq4000, NextSeq, and MiSeq sequencers 5.Chromium TM Single Cell DNA accessory Kit Magnetic beads station specifically used in CNV kit sample clean up. Contractors submitting a response to this Small Business Sources Sought notice shall possess the ability to achieve the salient government essential features of this potential requirement. Specifically, Offerors to this Small Business Sources Sought notice shall document evidence of being able to provide the subject supply that will allow for the essential features described herein to be accomplished. Delivery/Warranty Offerors should indicate an estimated delivery time in full after receipt of an order and any other training or warranty or software licenses applicable. Inspection and acceptance will be performed at NCATS located at 9800 Medical Center Drive, Rockville, MD 20852. CAPABILITY STATEMENTS SOUGHT Interested parties are expected to review this notice to familiarize itself with the requirements of this project. Failure to do so will be at your firm's own risk. Respondents must provide, as part of their response: (a) product, catalog, model, and/or part number(s); (b) product description; (c) all relevant information and documentation that the item(s) offered meets the salient physical, functional, or performance characteristics as specified in this announcement; (d) quantity; (e) shipping, handling, and/or installation instructions, and/or any special delivery conditions after receipt of an order. Respondents that believe that they are manufacturers or authorized re-sellers of the equipment specified in this announcement must provide, as part of their response: (a) product, catalog, model, and/or part number(s); (b) product description; (c) all relevant information and documentation that the item(s) offered meets the salient physical, functional, or performance characteristics as specified in the purchase description; (d) quantity; (e) estimated price or cost; (f) shipping, handling, and/or installation charges; and delivery date after receipt of order. The respondent must also provide their DUNS number, organization name, address, point of contact, and size and type of business (e.g., 8(a), HubZone, etc.) pursuant to the applicable NAICS code and any other information that may be helpful in developing or finalizing the acquisition requirements. The information submitted must be in outline format that addresses each of the elements of this requirement. A cover page and an executive summary may be included but is not required. The response is limited to ten (10) pages. The 10-page limit does not include the cover page, executive summary, or references, if requested. The response must include the respondents' technical and administrative points of contact, including names, titles, addresses, telephone and fax numbers, and e-mail addresses. Respondents must reference the subject announcement number on all correspondence related to this Small Business Sources Sought notice. The response must be submitted to Hunter Tjugum electronically, at hunter.tjugum@nih.gov, and be received prior to the closing date specified in this announcement. CONCLUDING STATEMENTS Disclaimer and Important Notes. This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization's qualifications to perform the work. Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After a review of the responses received, a pre-solicitation synopsis and solicitation may be published in Federal Business Opportunities. However, responses to this notice will not be considered adequate responses to a solicitation. Confidentiality. No proprietary, classified, confidential, or sensitive information should be included in your response. The Government reserves the right to use any non-proprietary technical information in any resultant solicitation(s).
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