SOURCES SOUGHT
66 -- Live Cell Imaging System with Super-Resolution Spinning Disk Confocal and Photostimulation Scanner
- Notice Date
- 3/4/2020 11:05:43 AM
- Notice Type
- Sources Sought
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- NATIONAL INSTITUTES OF HEALTH NIDA Bethesda MD 20892 USA
- ZIP Code
- 20892
- Solicitation Number
- HHS-NIH-NIDA-SSSA-20-004328
- Response Due
- 3/18/2020 12:00:00 PM
- Archive Date
- 04/02/2020
- Point of Contact
- Rashiid Cummins
- E-Mail Address
-
rashiid.cummins@nih.gov
(rashiid.cummins@nih.gov)
- Description
- This is a Sources Sought notice. This is NOT a solicitation for proposals, proposal abstracts, or quotations. The purpose of this notice is to obtain information regarding the availability and capability of all qualified sources to perform a potential requirement. This notice is issued to help determine the availability of qualified companies technically capable of meeting the Government requirement and to determine the method of acquisition.� It is not to be construed as a commitment by the Government to issue a solicitation or ultimately award a contract.� Responses will not be considered as proposals or quotes.� No award will be made as a result of this notice.� The Government will NOT be responsible for any costs incurred by the respondents to this notice.� This notice is strictly for research and information purposes only. Statement of Need and Purpose:� The iPSC neurodegenerative disease initiative (iNDI-CARD) requires a high content, super-resolution, high speed confocal microscope to study the effect of neurodegeneration-related mutations on critical cell biological processes. Background Information and Objective: This microscope will be used to identify converging mechanisms of disease across hundreds of isogenic iPSC-derived CNS cell types through advanced imaging and analysis techniques. Generic Name of Product: Live imaging system with spinning disk � Purchase Description/Salient Characteristics: All deliverables required under this contract shall be packaged, marked and shipped in accordance with Government specifications. At a minimum, all deliverables shall be marked with the contract number and Contractor name. The Contractor shall guarantee that all required materials shall be delivered in immediate usable and acceptable condition. Vendor must be able to provide a Live imaging system with spinning disk that meets these specifications: 25mm field of view:� This provides a very large field of view and ensures compatibility with large format CMOS sensors and higher data throughput. The transmitted and fluorescence illumination is engineered specifically for flatness across the 25mm field, which is advantageous for quantification and high throughput applications such as tiling. Increased motor speed and stability: Lowers latency and higher precision means increased speed during motorized operations. Compatibility with multiple imaging modalities: The widefield, TIRF, confocal and super-resolution imaging are all able to work together in one system.� The proposed system also includes a CSU-W1 spinning disk attachment and dedicated galvo scanner for photostimulation. Slide Express 2 120 slide capacity, robotic transfer, encoded XY stage with Joystick:� This is a high Speed linear encoded XY stage for high content acquisition. Piezo Z insert:� Piezo Z insert for rapid Z stacking for high resolution images during live cell imaging. Stage top incubation system:� Maintains and controls a stable environment for the 44 plates that are in que for transfer by the robot to the stage and to ensure healthy and viable cells. The generation 4 PFS system offers further improvements on the already unparalleled drift correction performance of the PFS3 system. These improvements include: Improved Z speed due to redesigned motors plus the measuring unit bolts directly mounted onto the body of the microscope to reduce mechanical load and improve Z stability. Includes a CSU-W1 SoRa spinning disk confocal scanner:� This system has an increased pinhole spacing (500 uM) reduces spatial crosstalk between adjacent pinholes. This improves clarity when imaging deep or through very dense or bright samples, relative to the CSU-X1 model.� Built in widefield bypass mode allows one camera to be used for both confocal and widefield modes allowing for faster and differential images of the living cells.� It has a dual integrated internal 10 position emission filter wheel with individual band emission filters.� With the High-power solid-state laser combiner provides 405, 488, 561 and 640nm lines with a second 405 output for photostimulation, essential for protein expression levels within the cells. Specialized SoRa emission disk with offset microlenses:� This is Coupled with a motorized intermediate magnification selector unit, these allow real-time super-resolution via optical pixel reassignment, which ultimately reduces emission PSF diameter at the detector plane. Seamless integration with NIS-Elements software:�� Allows easy switching between standard confocal, super-resolution and epi imaging, essential to support all applications in live cell imaging. Universal NIDAQ interface allows custom programming of analog and TTL devices for a wide range of custom device support for:� Light sources, XY and Z sample positioning devices.� It supports the pumps, injections systems as well as perfusion systems, all used for cell handling.� The interface also supports camera triggering for high speed and precision of external device control and synchronization. Comprehensive suite of acquisition and analysis features: It has a custom acquisition routine programming with JOBS module.� It has on-the-fly of post batch image analysis, usefully for end users and time management. This system has the 2D/3D deconvolution module for super resolution processing of the spinning disk data. Optimicroscan XY-galvo scanner for photostimulation:� This compact galvo scanner connects to the back of the microscope and utilizes a separate lightpath from the confocal system.� A beam combing mirror in a standard fluorescence cube allows simultaneous imaging and photostimulation with 405nm light. A 4 line laser launch is included in this configuration for stimulation capabilities with 405, 488, 561 and 640nm lasers, the latter three of which can be applied in a sequential stimulation configuration via rotation of a motorized cube turret. Dual Back-illuminated sCMOS detectors: The system includes quantity (2) BSI Prime back-illuminated scientific CMOS cameras. NIS-Elements supports this camera natively in single and dual configurations. The dual camera setup will be achieved with an integrated beamsplitter in the W1.� It has 95% peak qE means lower laser powers and shorter exposure times than standard 82% qE sCMOS detector, reducing the cells to be under extended exposure periods to powerful lasers. � This unit has a specialized SoRa emission disk with offset microlenses coupled with a motorized intermediate magnification selector unit:� This allows for real-time super resolution via optical pixel reassignment, which ultimately reduces emission PSF diameter at the detector plane. � Robotics and Devices:� 44 plate Incubator with transfer robot, bar code reader, 2 plate carriers; 44 Plate enclosure, transfer robot, bar code reader, two plate carriers; Bar Code printer and labels; Slide Express 2 120 slide capacity, robotic transfer, encoded XY stage, Joystick and x3 Cassettes and holders for 40 1x3"" slides.� These will support the high levels of samples that will be processed. The robotics system has the ability to do single plate or multi-well plates. Delivery Date: 90 days after receipt of purchase order Capability Statement/Information Sought: The capability statement should include 1) the total number of employees, 2) documentation of ability to provide the required equipment, 3) any contractor GSA Schedule contracts by which all of the requirements may be met, if applicable, and 4) any other information considered relevant to this program. The respondent must also provide their DUNS number, organization name, address, point of contact, and size and type of business (e.g., 8(a), HubZone, etc., pursuant to the applicable NAICS code and any other information that may be helpful in developing or finalizing the acquisition requirements. One (1) copy of the response is required and must be in Microsoft Word or Adobe PDF format using 11-point or 12-point font, 8-1/2� x 11� paper size, with 1� top, bottom, left and right margins, and with single or double spacing. The information submitted must be in an outline format that addresses each of the elements of the project requirement and in the capability statement /information sought paragraphs stated herein.� A cover page and an executive summary may be included but is not required. The response is limited to ten (10) page limit.� The 10-page limit does not include the cover page, executive summary, or references, if requested. The response must include the respondents� technical and administrative points of contact, including names, titles, addresses, telephone and fax numbers, and e-mail addresses. All responses to this notice must be submitted electronically to the Contract Specialist.� Facsimile responses are NOT accepted. The response must be submitted to Rashiid Cummins (Contract Specialist) at e-mail address Rashiid.Cummins@nih.gov. The response must be received on or before March 18th, 2020, 3:00pm, Eastern Time. �Disclaimer and Important Notes:� This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization�s qualifications to perform the work. Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After a review of the responses received, a presolicitation synopsis and solicitation may be published in Federal Business Opportunities. However, responses to this notice will not be considered adequate responses to a solicitation. Confidentiality: No proprietary, classified, confidential, or sensitive information should be included in your response. The Government reserves the right to use any non-proprietary technical information in any resultant solicitation(s).�
- Web Link
-
SAM.gov Permalink
(https://beta.sam.gov/opp/a3c5a60b1ea3460e9b7257d84b7c82b0/view)
- Place of Performance
- Address: Bethesda, MD, USA
- Country: USA
- Country: USA
- Record
- SN05579962-F 20200306/200304230147 (samdaily.us)
- Source
-
SAM.gov Link to This Notice
(may not be valid after Archive Date)
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