SOURCES SOUGHT
Q -- Maric Protocol Antibody Validation
- Notice Date
- 4/17/2020 11:31:52 AM
- Notice Type
- Sources Sought
- NAICS
- 621511
— Medical Laboratories
- Contracting Office
- NATIONAL INSTITUTES OF HEALTH NIDA Bethesda MD 20892 USA
- ZIP Code
- 20892
- Solicitation Number
- HHS-NIH-NIDA-SSSA-20-006611
- Response Due
- 5/4/2020 11:00:00 AM
- Archive Date
- 05/19/2020
- Point of Contact
- Karen Mahon
- E-Mail Address
-
karen.mahon@nih.gov
(karen.mahon@nih.gov)
- Description
- This is a Sources Sought notice. This is NOT a solicitation for proposals, proposal abstracts, or quotations. The purpose of this notice is to obtain information regarding the availability and capability of all qualified sources to perform a potential requirement. This notice is issued to help determine the availability of qualified companies technically capable of meeting the Government requirement and to determine the method of acquisition. �It is not to be construed as a commitment by the Government to issue a solicitation or ultimately award a contract.� Responses will not be considered as proposals or quotes.� No award will be made as a result of this notice.� The Government will NOT be responsible for any costs incurred by the respondents to this notice.� This notice is strictly for research and information purposes only. Background:� Degenerative changes in human brain, skeletal muscle, and choroid plexus are prominent features of aging and several common age-related diseases. The specific pathological derangements underlying these degenerative changes are incompletely defined. Multiplex immunostaining provides an novel opportunity to delineate specific pathological derangements associated with degenerative changes in these tissues, and to provide morphological, temporal and spatial context for these derangements. Detailed antibody characterization and validation is required in order to design and complete successful multiplex experiments. The objective of this project is to complete validation of antibodies needed to complete tissue-specific multiplex immunostaining protocols for human brain, skeletal muscle and choroid plexus. Purpose and Objectives: The purpose of this collaborative project is to use multiplex immunohistochemistry to label and classify the key cell types and degenerative phenotypes in Human Brain, Skeletal Muscle (including mitochondria and neuromascular junction), and Choroid Plexus specimens.� Such validation is essential for successful design and execution of multiplex imunostaining protocols.� Prior to using a given antibody in a multi round multiplex project, each antibody must undergo a detailed characterization and validation procedure using the Maric protocol, with precise microwave based incremental antigen retrieval.� This protocol requires an initial round of antigen retrieval, staining, and imaging, followed by 9 additional rounds of stripping, antigen retrieval, restraining, imaging, for each antibody.� This complex procedure must be completed on each antibody to ensure data quality and to optimize the planning and execution of the multiplex.� Project requirements: The contractor shall perform cellular and phenotypic classification on Human CNS and/or skeletal muscle specimens.� This includes the following: Each antibody must undergo detailed characterization and validation procedure using Maric protocol. � An initial round of antigen-retrieval, staining, and imaging, followed by 9 additional rounds of stripping, antigen retrieval, restaining, imaging, for each antibody. Anticipated period of performance: August 1, 2020 through July 31, 2021 Capability statement /information sought. Respondents must provide, as part of their responses, a capability statement that includes information regarding respondents�: (a) staff expertise, including their availability, experience, and formal and other training; (b) current in-house capability and capacity to perform the work; (c) prior completed projects of similar nature; (d) corporate experience and management capability; and (e) examples of prior completed Government contracts, references, and other related information. The respondent must also provide their� DUNS number, organization name, address, point of contact, and size and type of business (e.g., 8(a), HubZone, etc., pursuant to the applicable NAICS code and any other information that may be helpful in developing or finalizing the acquisition requirements. One (1) copy of the response is required and must be in Microsoft Word or Adobe PDF format using 11-point or 12-point font, 8-1/2� x 11� paper size, with 1� top, bottom, left and right margins, and with single or double spacing. The information submitted must be in and outline format that addresses each of the elements of the project requirement and in the capability statement /information sought paragraphs stated herein.� A cover page and an executive summary may be included but is not required. The response is limited to ten (10) page limit.� The 10-page limit does not include the cover page, executive summary, or references, if requested. The response must include the respondents� technical and administrative points of contact, including names, titles, addresses, telephone and fax numbers, and e-mail addresses. All responses to this notice must be submitted electronically to the Contract Specialist and Contracting Officer.� Facsimile responses are NOT accepted. The response must be submitted to Karen Mahon at e-mail address Karen.Mahon@nih.gov. The response must be received on or before May 4, 2020, at 2:00 pm Eastern Time. ��Disclaimer and Important Notes:� This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization�s qualifications to perform the work. Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After a review of the responses received, a presolicitation synopsis and solicitation may be published in Federal Business Opportunities. However, responses to this notice will not be considered adequate responses to a solicitation. Confidentiality: No proprietary, classified, confidential, or sensitive information should be included in your response. The Government reserves the right to use any non-proprietary technical information in any resultant solicitation(s).�
- Web Link
-
SAM.gov Permalink
(https://beta.sam.gov/opp/ef8d4fb38a1f476485e76678fae76434/view)
- Record
- SN05625245-F 20200419/200417230148 (samdaily.us)
- Source
-
SAM.gov Link to This Notice
(may not be valid after Archive Date)
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