MODIFICATION
Q -- Protein Mass Spectrometry Analysis
- Notice Date
- 9/2/2020 2:02:08 PM
- Notice Type
- Combined Synopsis/Solicitation
- NAICS
- 541380
— Testing Laboratories
- Contracting Office
- NATIONAL INSTITUTES OF HEALTH NIDA Bethesda MD 20892 USA
- ZIP Code
- 20892
- Solicitation Number
- HHS-NIH-NIDA-NIA-CSS-20-007469
- Response Due
- 9/10/2020 9:00:00 AM
- Archive Date
- 12/31/2020
- Point of Contact
- Debra C. Hawkins, Phone: 3018277751
- E-Mail Address
-
Debra.Hawkins@nih.gov
(Debra.Hawkins@nih.gov)
- Description
- (i) This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in Subpart 12.6 as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; proposals are being requested and a written solicitation will not be issued. (ii) The solicitation number is HHS-NIH-NIDA-NIA-CSS-20-007469 and the solicitation is issued as a Request for Quotation (RFQ). This acquisition is for a commercial item or service and is conducted under the authority of the Federal Acquisition Regulation (FAR) Part 13�Simplified Acquisition Procedures; FAR Subpart 13.5�Simplified Procedures for Certain Commercial Items; and FAR Part 12�Acquisition of Commercial Items and is not expected to exceed the simplified acquisition threshold. The solicitation is issued as non-competitive.�� �(iii) This solicitation incorporates the provisions and clauses are those in effect through Federal Acquisition Circular (FAC) 2020-08, effective August 13, 2020. �(iv) The associated North American Industry Classification System (NAICS) code: 541380�Testing Laboratories. Size Standard: $16.5 Million.� (v) Protein Mass Spectrometry Analysis (vi) Background / Purpose / Objective:�� The National Institute on Aging (NIA), requires Protein Mass Spectrometry Analysis for the detection of the 14-3-3 protein binding partners in the heart targeted AdCy8 overexpression mouse strain. The purpose of this project is to study the role of the 14-3-3 protein partners in order the elucidate their pathways and their mechanisms in heart cell survival, cell renual and cell mitosis and its regulation.� The overexpression of adenylyl cyclase in the mouse heart produces a strong beating heart with the upregulation of many pathways. The 14-3-3 protein is one of these proteins that emerge as central for the heart survival and regulation of the cell cycle and mitosis check points.�� Here we plan to analyze the role of the 14-3-3 protein by detecting its binding partners in the AdCy8 overexpression model. This analysis will be done through the use of a special method called immunoprecipitation (IP) followed by mass spectrometry analysis (MS) and comparison of the results with the wild type mouse model. Anticipated period of performance / delivery date:� The Government anticipates the period of performance as five (5) to ten (10) weeks after award. Project Requirements:� Independently and not as an agent of the Government, the Contractor shall furnish all the necessary services, qualified personnel, material, equipment, and facilities, not otherwise provided by the Government as needed to perform the�specific requirements below. Specific Requirements:� The contractor shall conduct protein mass spectrometry analysis for the detection of the 14-3-3 protein binding partners in the heart targeted AdCy8 overexpression mouse strain as follows: �(1) IP tests in control and AC8TG tissues; (2) anti IP 14-3-3 analysis; (3) data analysis and comparison; and (4) provide a report on each step of the analysis and detail the results in a spreadsheet format. The protein mass spectrometry services perform method development and testing of pan 14-3-3 on 24 samples. The immune-precipitation requires the use of a specific antibody against the 14-3-3 protein. This antibody needs to recognize every member of the 14-3-3 protein and be suitable for immono-precipitation. Mass spectroscopy run for the detection and the recognition of the 14-3-3 protein binding partners.� Immuno Precipitation analysis (IP) to be performed on 24 samples. Labor category: Senior Researcher. Government Responsibilities: The government will provide 24 AC8 samples to the contractor to perform the Protein Mass Spectrometry Analysis. Delivery or Deliverables:� Interim and final report on each step of the analysis and detail the results in a spreadsheet format. Reporting Requirements: Final Report detailing the results of the analysis. This requirement is a follow-on to protein mass spectrometry analysis for the HAC8 research project conducted by MS Bioworks, LLC, 3950 Varsity Drive, Ann Arbor, Michigan 48108. �(vii) Government's Anticipated Delivery/Period of Performance: The Government anticipates the period of performance as five (5) to ten (10) weeks after receipt of order (ARO), September 2020 through January 31, 2021. Delivery Point: FOB Destination. Delivery Location: The National Institute on Aging (NIA), Baltimore, Maryland 21224. The successful offeror shall coordinate delivery arrangements with the customer prior to shipment. The Loading Dock hours are as follows: 8:00 am to 4:30 pm Eastern Time, Monday through Friday except on Federal holidays. All products delivered must contain a Packing Slip (with serial numbers as applicable). �(viii) The provision at FAR clause 52.212-1, Instructions to Offerors - Commercial Items, applies to this acquisition. (ix) The provision at FAR clause 52.212-2, Evaluation - Commercial Items, applies to this acquisition. The Government will award a contract resulting from this solicitation to the responsible offeror whose offer conforming to the solicitation will be most advantageous to the Government, price and other factors considered. The factors used to evaluate offers are the unique technical and performance capabilities of product and cost. Technical and past performance, when combined, are significantly more important than cost or price.� (x) Offerors are advised to include a completed copy of the provision at FAR clause 52.212-3, Offeror Representations and Certifications - Commercial Items, with its offer. (xi) FAR clause at 52.212-4, Contract Terms and Conditions - Commercial Items, applies to this acquisition. (xii) FAR clause at 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders - Commercial Items, applies to this acquisition. (xiii) There are no additional contract requirement(s) or terms and conditions applicable to this acquisition. (xiv) Defense Priorities and Allocations System (DPAS) and assigned rating, is not applicable to this solicitation. (xv) All quotations or offers are due 12:00 Noon, Eastern Time, on September 10, 2020, and must reference the solicitation number noted herein.� Quotations or offers must be submitted electronically to the contact person identified herein at debra.hawkins@nih.gov.� Fax responses will not be accepted. (xvi) For information regarding this solicitation contact: Debra C. Hawkins Contractor, Acquisition Policy Analyst II, at debra.hawkins@nih.gov.
- Web Link
-
SAM.gov Permalink
(https://beta.sam.gov/opp/22e157a556bc46c9bd3fc1c7ff865884/view)
- Place of Performance
- Address: Baltimore, MD 21224, USA
- Zip Code: 21224
- Country: USA
- Zip Code: 21224
- Record
- SN05783278-F 20200904/200902230134 (samdaily.us)
- Source
-
SAM.gov Link to This Notice
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