Loren Data's SAM Daily™

SAMDAILY.US - ISSUE OF JUNE 30, 2022 SAM #7517
SOURCES SOUGHT

66 -- CHEMIDOC - HERRLICH (VA-21-00075743)

Notice Date

6/28/2022 6:24:06 PM
 

Notice Type

Sources Sought
 

NAICS

334516 — Analytical Laboratory Instrument Manufacturing
 

Contracting Office

RPO EAST (36C24E) PITTSBURGH PA 15212 USA
 

ZIP Code

15212
 

Solicitation Number

36C24E22Q0170
 

Response Due

7/5/2022 9:00:00 AM
 

Archive Date

08/04/2022
 

Point of Contact

Matenoa Taufa, Contract Specialist, Phone: 412-822-3434
 

E-Mail Address

matenoa.taufa@va.gov
(matenoa.taufa@va.gov)
 

Awardee

null
 

Description

Summary: This is a Sources Sought notice and not a request for quotes. This request is solely for the purpose of conducting market research to enhance VHA s understanding of the market s offered products, services and capabilities. The Government will not pay any costs for responses submitted in response to this Sources Sought.  This Sources Sought notice provides an opportunity for respondents to submit their notice of ability, and their available products and services in response to the requirement described below. Vendors are being invited to submit information relative to their potential of fulfilling the requirement below, in the form of a capability response that addresses the specific requirement identified in this Sources Sought.    The Veterans Health Administration (VHA) is seeking a vendor to provide the following products and/or services below. This Sources Sought is to facilitate the Contracting Officer s review of the market base, for acquisition planning, size determination, and procurement strategy.  The Department of Veterans Affairs, St. Louis VA Medical Center, is in need of a Biorad ChemiDoc MP Imaging System and Biorad Trans-Blot® Turbo Transfer System needed for measurements of protein levels to validate changes in gene expression and to quantify changes in these studies.   Technical Specifications/ salient characteristics Salient Characteristics Chemi-Doc Imaging System Required for imaging western blot and agarose gels and enable quantification of data derived. Feature set/Technical capabilities and standards: 2,750 H x 2,200 V pixels, 6.05 megapixels 70% quantum efficiency (QE) at 425 nmmaximum QE of 75% at 525 nm/ 75 50% QE at 525 800 nm f/0.95 fast lens 16-bit data /acquisition/4.54 x 4.54 m pixel size : Low read noise, 6e Low dark current, 0.002 e /p/s at 15°C Peltier cooling to 15°C, absolute: White Sample Tray for trans-white illumination; use with colorimetric stains, such as Coomassie and silver stains. Flexible and automated image exposure setting for all compatible applications. Ensure the optimal exposure time to obtain the best dynamic range for all applications. Optimal auto-exposure for chemiluminescence: produces an image with full dynamic range. This setting is a good choice when performing quantitative analysis of the blot Rapid auto-exposure for chemiluminescence: acquires an image with a limited dynamic range using a shorter exposure time Manual exposure is available to override automated imaging Software-suggested bin setting based on defined imaging area to achieve publication-quality images. Image Lab Touch Software takes the guesswork out of estimating the shortest exposure time for obtaining high-resolution images (175 microns per pixel). Several image export options with Image Lab. Users can export images to a flash drive, external hard drive, and shared folder on a network drive or stand-alone computer. Users can export images in several file formats (Image Lab SCN/MSCN, 16-bit TIFF, 8-bit TIFF, JPG) suitable for their purposes, such as analysis and publication. Illumination sources include: Trans-ultraviolet: 302 nm excitation Epi-white Trans-white (requires White Sample Tray) Trans-blue: 450 490 nm excitation (requires Blue Sample Tray) Epi-blue: 460 490 nm excitation Epi-green: 520 545 nm excitation Epi-red: 625 650 nm excitation Epi far red: 650 675 nm excitation Epi near infrared: 755 777 nm excitation Standard 590/110 nm filter and chemiluminescence filters: 518 546 nm filter for blue-excitable fluorophores and stains 577 613 nm filter for green-excitable fluorophores and stains 675 725 nm for red-excitable fluorophores and stains 700 730 nm filter for far red excitable fluorophores and stains 813 860 nm for near infrared excitable fluorophores and stains. Key points: High-efficiency charge-coupled device (CCD) with low read and dark noise provides reduced background and enhanced sensitivity to match the sensitivity of X-ray film (gold standard). Deeply cooled sensor allows longer integration time to detect weak signals. High-resolution CCD ideal for resolving closely spaced bands on a gel or blot. Quantitative imaging system over at least 4 orders of linear dynamic range and to allow users to gather maximum information from a blot that has both intense and weak signals in a single image. No need for a separate transilluminator with Ultraviolet (UV) light. System that protects samples from ultraviolet damage and allows external visualization of DNA samples without a UV shield. The different available illumination sources allow unprecedented versatility in multicolor detection. Compatible with stain-free technology for gel and blot protocols. Technology functionality allowing researchers quality control (QC) validation of their results at each stage of the blotting process. The ChemiDoc Imaging Systems provide total protein normalization and stain-free technology as an alternative to normalization with single housekeeping proteins (HKPs). Stain-free total protein normalization can be used to validate HKPs or may replace HKPs entirely, simplifying the western blot workflow and allowing for probing another antigen of interest on the same western blot Smart Tray Technology: Automatic recognition of application-specific tray and adjustment of imaging Parameters and software options Autofocus: Pre-calibrated focus for any zoom setting or sample height Auto-exposure: Two user-defined modes (rapid or optimal auto-exposure) for all blot and gel Imaging applications Image flat fielding: Dynamic pre-calibrated and optimized for each application Touch-screen: Multitouch capability 12.1 display Maximum image area: 21 x 16.8 cm Detector: Cooled CCD, 6 megapixels Illumination sources: Trans-UV, 302 nm excitation, Epi-white (requires White Sample Tray) Trans-blue 450-490 nm excitation (requires Blue Sample Tray) Detection: Chemiluminescence detector, 590/110 nm standard emission filter for protein and DNA gel and blot imaging Data output: 16-bit or 8-bit SCN, TIFF, JPEG files Salient Characteristics -Transfer System: Required for protein transfers from SDS page protein gels onto membrane for the staining with antibodies and subsequent visualization in the Biorad ChemiDoc MP Imaging System. Combination of traditional blotting techniques with modern filter paper and buffers, allowing rapid transfer of proteins from gels to membranes with minimal preparation time. System integrates a high amperage power supply that directs current between a built-in platinized titanium anode and a stainless steel cathode. This feature enables highly efficient and rapid transfer in as little as 3 minutes. System in a ready-to-use format that allows researchers to obtain their results faster and easier, with reproducibility. Rapid transfer  transfers mini or midi gels in as little as 3 min High throughput  can transfer 1 4 mini or 1 2 midi gels in a single run Greater transfer efficiency  higher transfer efficiency compared to other transfer methods Flexible design  allows user to customize transfer conditions and is compatible with traditional semi-dry consumables Environmentally friendly  environmentally safe consumables eliminate disposal cost Additional Items Required: Additional White Light Conversion Screen (White sample tray WDT; added to the Bio-Rad quote), for viewing Coomassie, silver stain, and other colorimetric gel stains. StarBright Blue 700 Goat Anti-Rabbit IgG, 80 µl #12004162 Pkg of 1, lyophilized goat anti-rabbit IgG conjugated with StarBright Blue 700 Fluorophore; reconstitute to 80 µl hFAB Rhodamine Anti-Tubulin Primary Antibody 40 µl #12004166U Pkg of 1, lyophilized rhodamine-conjugated anti-tubulin human Fab fragment; reconstitute to 40 µl Precision Plus Protein Unstained Protein Standards, Strep-tagged recombinant, 1 ml #1610363 1 ml, unstained mixture of ten Strep-tagged, recombinant proteins (10 250 kD), including three reference bands (25, 50, 75 kD), 100 applications Clarity Max Western ECL Substrate, 100 ml #1705062 Pkg of 1, contains 50 ml Clarity Max Western Peroxide Reagent and 50 ml Clarity Max Luminol/Enhancer Reagent 4 20% Mini-PROTEAN® TGX Stain-Free Protein Gels, 15 well, 15 µl #4568096 Pkg of 10, 4 20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells Trans-Blot® Turbo Transfer System #1704150 additions includes base, 2 cassettes to hold 1 2 midi or up to 4 mini blotting sandwiches, blot roller Trans-Blot Turbo Mini 0.2 µm Nitrocellulose Transfer Packs #1704158 Pkg of 10, 7 x 8.5 cm, precut blotting transfer pack, includes filter paper, buffer, 0.2 µm nitrocellulose membrane, for use with Trans-Blot Turbo Transfer System Trans-Blot Turbo Mini 0.2 µm PVDF Transfer Packs #1704156 Pkg of 10, 7 x 8.5 cm, precut blotting transfer pack, includes filter paper, buffer, 0.2 µm nitrocellulose membrane, for use with Trans-Blot Turbo Transfer System White Sample Tray for ChemiDoc MP/ChemiDoc Imaging Systems #12003026 Pkg of 1, sample tray for gels and blots stained with Coomassie Blue, copper, silver, or zinc stains 4. Delivery:  Department of Veterans Affairs, St Louis 915 North Grand Boulevard St. Louis, MO 63106-1621  5. Installation:   Installation and demonstration are not required.    6. Information Technology:  IT support requirements are needed for this equipment.    RESPONSE COMMITMENT    I. Response:    A. Capability statement and with general understanding of the industry and current knowledge and subcontract networks that would be used to support the requirement as identified herein. Provide small business information for your company.  B. Submittals furnished will not be returned to the sender. No debriefs will be conducted. Eligibility in participating in a future acquisition does not depend upon a response to this notice.    II. Timeline:   A. This request will close on stated date within the Government Point of Entry (GPE).    Notes:   This Sources Sought is for planning purposes only to gain knowledge of potential qualified sources and their size classification (large business, small business, 8(a), SDVOSB, etc.), relative to NAICS code 334516. Responses to this source sought will be used by the Government to make appropriate acquisition decisions. This notice is not a request for competitive proposals; however, any responsible source who believes it is capable of meeting the requirement and who is the Original Equipment Manufacturer (OEM), authorized dealer, authorized distributor or authorized reseller of all the items listed above, may submit a capability statement to the contracting office than no later than Tuesday, July 05, 2022, 12:00 PM EST. Interest/capability statements may be sent to Matenoa Taufa Matenoa.taufa@va.gov. No telephone responses will be accepted.
 

Web Link

SAM.gov Permalink
(https://sam.gov/opp/98ee2c67ab484827a14e0a81590a85fa/view)
 

Place of Performance

Address: Department of Veterans Affairs, St Louis Washington University School of Medicine 425 S. Euclid Avenue, St. Louis 63110, USA

Zip Code: 63110

Country: USA
 

Record

SN06372435-F 20220630/220628230131 (samdaily.us)
 

Source

SAM.gov Link to This Notice
(may not be valid after Archive Date)

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