SPECIAL NOTICE
B -- Larvel DNA Identification Services
- Notice Date
- 8/3/2022 12:21:35 PM
- Notice Type
- Special Notice
- NAICS
- 541690
— Other Scientific and Technical Consulting Services
- Contracting Office
- DEPT OF COMMERCE NOAA KANSAS CITY MO 64106 USA
- ZIP Code
- 64106
- Response Due
- 8/12/2022 3:00:00 PM
- Point of Contact
- Amy Gilliland, Phone: 2404601033
- E-Mail Address
-
amy.gilliland@noaa.gov
(amy.gilliland@noaa.gov)
- Description
- NFFN76002202354AAG ***NOAA�s Acquisition & Grants Office, Western Acquisition Division-Boulder, Southeast Fisheries Science Center (NEFSC), Population and Ecosystems Monitoring (PEM) Division �intends to negotiate, on a sole source basis, under authority of FAR 13.106-1(b)(1), with Texas A&M University at Galveston, to utilize genotyping methods via high resolution melting analysis (HRMA) in combination with the non-invasive larval DNA isolation protocol �Shake and Stew� techniques to refine taxonomic resolution of Southeast Area Monitoring and Assessment Program (SEAMAP) larval specimens and create species-specific larval abundance indices for the relevant species. Services will be conducted at the Universities Ocean and Coastal Studies Building, room 247, 1001 Texas Clipper Rd. Galveston, TX 77554. ***The sole source determination is based on the Southeast Fisheries Science Center (SEFSC) requirement to provide positive identifications of over 17,000 larval thunnus specimens in the SEAMAP archive. The objective of the proposed work is to utilize genotyping methods via high resolution melting analysis (HRMA) in combination with the non-invasive larval DNA isolation protocol �Shake and Stew� techniques to refine taxonomic resolution of Southeast Area Monitoring and Assessment Program (SEAMAP) larval specimens and create species-specific larval abundance indices for the relevant species. Individual specimens from the SEAMAP archive will be processed using the �Shake and Stew� method where DNA from the epithelial cells of fish larva will be released into the preservative fluid. DNA from each individual will be directly isolated from the cells suspended in the preservation fluid using a heated alkaline solution. The molecular technique of HRMA in combination with the non-invasive larval DNA isolation protocol �Shake and Stew� developed by Dr. Alvarado-Bremer for analyzing the data is not available from other sources as per communication with other NOAA Fisheries labs and research of recent publications in peer reviewed journals. Other laboratories provide services to genotype larval specimens; however, the methods used to extract DNA are destructive. The methods can range from removal of an eyeball or a fin clip, but in the case of small larvae, such as those found in the SEAMAP archive, the whole specimen has to be destroyed in order to get ample amounts of DNA. This can preclude the use of the larvae from future research such as use in describing morphological characteristics leading to improvement of larval species identification, measuring larval growth, stable isotope analysis, or feeding studies. Similar work by several Fisheries Science Center laboratories to genotype larvae for positive identifications has proven successful; however, the larvae have been damaged or consumed entirely by the methods. The collaboration with the University and specifically Dr. Alavardo-Bremer will continue to enhance the scientific value of the SEAMAP archived specimens.� Use of other facilities/laboratories to genotype specimens for this project cannot provide the assurance of MEFGEN that specimens will be returned undamaged back to the SEAMAP Archive. It is extremely important that specimens remain as undamaged as possible due to the larvae being part of the 38-year old museum collection at the SEAMAP archive, which is accessed by government and academic researchers from around the world. � The value to the Government is excellent. Overall cost and the condition of the larvae after the genotyping method is critical in determining the source for this molecular analysis. As part of a university system, Dr. Alvarado-Bremer has the equipment and technical assistance needed to perform the work. In order to do similar work, the equipment and personnel training needed to perform the tasks could cost over $200,000 per communication with NOAA colleagues. The Period of Performance is a Date of award through 12 months with one (1) 12 month option period. ***THIS NOTICE OF INTENT IS NOT A REQUEST FOR COMPETITIVE PROPOSALS AND NO SOLICITATION PACKAGE IS AVAILABLE. HOWEVER, FIRMS THAT BELIEVE THEY CAN FULLY MEET THE GOVERNMENT'S REQUIREMENTS MAY SUBMIT SUBSTANTIATING DOCUMENTATION IN WRITING TO THE IDENTIFIED POINT OF CONTACT WITHIN 15 DAYS AFTER PUBLICATION OF THIS SYNOPSIS. SUCH DOCUMENTATION WILL BE EVALUATED SOLELY FOR THE PURPOSE OF DETERMINING WHETHER OR NOT TO CONDUCT THIS PROCUREMENT ON A COMPETITIVE BASIS. A DETERMINATION BY THE GOVERNMENT NOT TO CONDUCT A COMPETITIVE PROCUREMENT, BASED UPON RESPONSES TO THIS NOTICE, IS SOLELY WITHIN THE DISCRETION OF THE GOVERNMENT. ORAL COMMUNICATIONS ARE NOT ACCEPTABLE IN RESPONSE TO THIS NOTICE.
- Web Link
-
SAM.gov Permalink
(https://sam.gov/opp/c29ba9a547584d6b9531d8ba2b1f4e7b/view)
- Place of Performance
- Address: Galveston, TX 77554, USA
- Zip Code: 77554
- Country: USA
- Zip Code: 77554
- Record
- SN06412094-F 20220805/220803230108 (samdaily.us)
- Source
-
SAM.gov Link to This Notice
(may not be valid after Archive Date)
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