VA Medical Center, Contract Office, 2215 Fuller Road, Ann Arbor, MI 48105

65 -- INCA++ CALCIUM IMAGING SYSTEM SOL 506-83-96 DUE 030696 POC Contact Point, BETTY GROSS, (313)930-5584, Contracting Officer, Contracting Office, (313)930-5580, Additional Contact, Contracting Office, (313)930-5580 THIS MEDICAL CENTER INTENDS TO NEGOTIATE A CONTRACT WITH INTRACELLULAR IMAGING INC, THE SYSTEM INCLUDES ALL REQUIRED HARDWARE AND SOFTWARE, CONSISTING OOF FOLLOWING ITEMS: HARDWARE: IMAGE PROCESSING COMPUTER : INTEL PENTIUM 90MHz MICROPROCESSOR WITH A CORECO OCMX IMAGE ANALYSIS BOARD, 16 MB of RAM, 1 G byte hard disk with 256k cache, 1.44 MB 3.5'' Teac floppy drive, quad speed CD-ROM, color graphic accelerator card, and 15'' SVGA noninterlaced monitor. (ii) NIKON TMS microscope includes trinocular head, phase optics, for epifluoresence illumination Microscope includes trinocular head, phase optics, 10x phase contrast objective, and mecanical stage with 60mm and petri dish holders. Also included is a choice of 60x or 40x epifluorescence non-phase contrast objective. (iii) 300 att compact Xenon are illuminator with self-contained power supply. (iv) Computer controlled filter changer and shutter unit containing 340nm and 380nm band bass filters for fura-2 fluorescence and liquid light guide and adapter for interfacing to a Nikon Diaphot microscope. (v) low light level, integrating CD monochrome video camera with microscope interfacing relay lense. (vii) All necessary cables and connectors, (ix) Full 90 day warranty on parts and labor. SOFTWARE: The software integrates the hardware components into a single, turnkey system and provides the following capabilities: (i) High resolution (512 x 480) visualization and spatial analysis of intracellular calcium. (ii) quantitative analysis of calcium in user defined, non-geometric regions of interest. (iii) Options for calculation and graphical analysis of calcium during the course of an experiment in defined regions of interest. (iv) Easy creation of pseudocolor tables for assignment of colors to gray level ranges. (v) Sequential playback of images for animation of changes in intracellular calcium over time. (vi) creation of annotated montages from selected images. (vii) Option for single wavelength analysis using non-ratiometric dyes (e.g. Fluo-3). OPTIONAL ITEMS: GROONY FLUORESCENCE OPTICS; These optics substitute for the Makler fluoresence optics that are standard with the system. Groony optics offer the advantage of a wider range of objectives, especially with regard to phase optics and a greater degree of freedom in turret movement for changing objectives. COMPUTER: Substitution of 17'' Computer Monitor; Additional 16 MB RAM. FILTER SETS (Specify desired dye or wavelengths) Dual wavelength set: 2 excitation filters in holder, and filter cube with emission filter and dichroic mirror. Single wavelength set: but with only 1 excitation filter. OBJECTIVES: 55x fluorite (oil), 100x fluorite (oil), 10x Nikon Fluorphase, 20x Nikon Flurophase, 100x Nikon Flurophase (oil). COLOR PRINTER: CANON BUBBLEJET BJ 4000 color printer. Incuding onsite installation and training at no additional cost. GOVERNMENT REQUIRES A 30 TESTING PERIOD WITH WARRANTY TO COMMENCE THEREAFTER. ALL RESPONSIBLE SOURCES MAY SUBMIT A BID WHICH WILL BE CONSIDERED BY THE AGENCY. CONTRACT AWARD IS SUBJECT TO THE AVAILABILITY OF FY 1996 FUNDS. SEE NUMBER Note 22. (0046)

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