USDA, APHIS, Field Servicing Office, Butler Square, Fifth Floor, 100 North Sixth Street, Minneapolis, MN 55403

65 -- TUBERCULIN SOL 70-M-APHIS-96 DUE 062496 POC POC: Jeanne V. Lewis is the Contracting Officer. She can be reached at Area Code (612) 370-2109 The USDA, Animal and Plant Health Inspection Service, (APHIS), Veterinary Services (VS), Riverdale, Maryland, intends to acquire Tuberculin, PPQ Bovis Intradermic in 10 ml vials. Veterinary Services requires an estimated 250,000 ml at PPD Bovis Tuberculin per year. The PPD Bovis Tuberculin is required as a diagnostic test reagent in the national eradication program for bovine Tuberculosis. It is a product derived from the culture of the AN-5 strain of Mycobacterium bovis in a modified Reid's media for 8-12 weeks in a constant temperature incubator equipped with a device to increase the relative humidity. These cultures are grown as floating cultures to maximize the yield of tuberculin. The 8-12 week culture is heat killed with steam under pressure at 121 degrees C for 30 minutes. The culture media is filtered, preserved in a 5 percent phenol solution, concentrated to 2 percent of the original volume, and precipitated in ammonium sulfate to yield a product with a protein concentration of 1 mg per ml. Each serial lot of tuberculin shall be tested for purity, safety, potency, and special chemical characteristics in accordance with the conditions presented in Title 9, Code of Federal Regulations (9CFR) Parts 113.203, 113.3 or in Section V of the approved outline of production, which is on file with USDA, APHIS, VS. We are interested in companies that have the capability to provide PPD Bovine Tuberculin. The contract will be for a one-year period with two option years. This is a fixed price requirements program. F.O. B. destination. The solicitation is to be released on or about May 15, 1996. For a copy of the solicitation submit written or FAXed request stating name, address, telephone number and solicitation number. Faxed requests to ''Contracting Section'' at (612) 370-2083. Telephone requests will not be accepted. All responsible sources may submit an offer which will be considered. A copy of the solicitation will be available in this office for review. (0116)

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