USDA, APHIS, Field Servicing Office, Butler Square, Fifth Floor, 100 North Sixth Street, Minneapolis, MN 55403

99 -- TUBERCULIN SOL S-M-APHIS-1-96 DUE 072696 POC POC: The Contracting Officer is Jeanne Lewis. She can be reached at area code (612) 370-2109 This synopsis is not in support of an existing solicitation. It is a sources sought synopsis for the purpose of locating additional firms that have products that meet the requirements of the United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service (APHIS), Veterinary Services (VS), for supplying an estimated 250,000 ml of PPD Bovis Tuberculin per year. The PPD Bovis Tuberculin is required as a diagnostic test reagent in the national eradication program for bovine tuberculosis. It is a product derived from the culture of the AN-5 strain of Mycobacterium bovis in a modified Reid's media for 8-12 weeks in a constant temperature incubator equipped with a device to increase the relative humidity. THese cultures are grown as floating cultures to maximize the yield of tuberculin. The 8-12 week culture is heat killed with steam under pressure at 1212 degrees C for 30 minutes. The culture media is filtered, preserved in a 5 percent phenol solution, concentrated to 2 percent of the original volume, and precipitated in ammonium sulfate to yield a product with a protein concentration of 1 mg per ml. Each serial lot of tuberculin shall be tested for purity, safety, potency and special chemical characteristics in accordance with the conditions prescribed in title 9 code of Federal Regulations (9CFR) Parts 113.203, 113.3 or in Section V of the approved outline of production, which is on file with USDA, APHIS, VS. We are interested in companies that have the capability to provide the PPD Bovine Tuberculin for future requirements. Interested firms may contact Jeanne Lewis, Contracting Officer, at area code (612) 370-2109 for additional information. (0179)

Loren Data Corp. http://www.ld.com (SYN# 0430 19960628\99-0005.SOL)