U.S. Environmental Protection Agency, 26 W. Martin Luther King Dr., Cincinnati, OH 45268

A -- 12 REACTOR, COMPUTERIZED RESPIROMETER SYSTEM SOL RFQ OH-97-00270 DUE 081597 POC Kathleen Roe, phone (513) 366-2144, fax (513) 366-2107 WEB: Office of Acquisition Management -- Cincinnati Contracts Management Division, This procurement will not appear on the web site.. E-MAIL: U.S. EPA -- Cincinnati Contracts Management Division, roe.kathleen@epamail.epa.gov. The U. S EPA National Risk Management Research Laboratory has a requirement for a computerized, automated respirometer is required for conducting experiments that monitor continuously the respiratory activity of bacteria consuming crude oil hydrocarbons over periods of weeks to months. The system shall be able to monitor oxygen uptake continuously over time while bacteria degrade oil or hydrocarbons in aqueous or solid media. The data shall be recorded continuously via a personal computer hookup using appropriate software. The software shall be able to display the oxygen uptake curves on a color monitor or derivatives of the uptake curves (i.e. uptake rates), and the operator shall be able to display the oxygen uptake curves for any or all flasks in operation. The respirometer shall be equipped with an alkaline trap for measurement of carbon dioxide evolution evolved into the headspace. Each flask in the respirometric system shall be independent of all other flasks, and the headspaces shall be kept completely separate at all times to prevent bacterial contamination or escape of volatile components from the oil under study. The respirometer shall be equipped with an accurate water bath to control temperature within the range 15 to 50 C. Response date is approximate. Please submit your request for an RFQ in writing via e-mail or fax to the POC listed above. (0197)

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