Acquisitions Management Operations Branch, NIAID, 6003 Executive Blvd, Solar Building, Room 1C38, Contracts Section, Bethesda, MD 20892-7605

66 -- 3826 AKTAFPLC SYSTEM SOL NO1-#9810 DUE 090898 POC Linda Taylor,PA 301-402-2282/Carolyn Sentz, Contracting Officer,301-402-2282 This is a combined synopsis/notice of intent to procure the Amersham AKTA FPLC Liquid Chromatography System Cat# 13-3300-01 combined with AKTA FPLC Cat#18-1118-67. This system has the ability to purify biomolecules from complex starting materials derived from cell lysates, tissue homogenates recombinant protein expression systems and other sources. The system will be able to perform separations based on a variety of sample characteristics, such as molecular weight, net surface charge, hydrophobicity, while retaining biological activity. This system consist of the following items; (a) Amersham Motor Valve PV-908 Cat#18-1108-41 (b) Amersham pH Flow Cell with Dummy Cat# 18-1112-92; (c) Amersham pH Electrode pH/C-900 Cat#18-1111-26; (d) Amersham Sample Pump p-910 Cat# 18-113-86; (e) Tube Rack 12mm Delta (f) Compaq Deskpro 2000 system control unit Cat# 13-3188-01; and HP deskjet 692c w/cable Cat#13-3043-01. This instrument will be used in setting up research facility and was selected as an upgrade to an existing instrument. It has the following unique features for sole source justification. (1) Has the ability to purify biomolecules from complex starting materials based on a variety of characteristics such as molecular weight, net surface charge, hyrophobicity, while retaining biological activity. (2) Has a flow rate range of 0.05-20ml/min and pressure ranges from 0-5mpa with high accuracy and precision in gradient fromation. (3) Has combination/integrated in-line monitoring of uv (0.01 to 5.0 AUFS), conductivity (1uS/cm to 999mS/cm), and ph (range 0-14 in 0.1 unit accuracy) all in one unit for lowest possible system dead vol. (4) Has motorized PV-908 valves that accommodate up to seven columns upgraded for compatibility from previous system plus one column bypass for washes and calibrations, facilitating column cleaning, storage, and use of columns. (5) Fraction collection by automatic peak detection or collection by vol. (6) System controller and unique software capability to monitor 3 pumps, 9 motorized valves, a fraction collector, a gradient mixer, a UV monitor, a conductivity monitor, a pH monitor, and 3 air sensors. The provision at FAR 52.212-1, Instructions to Offerors Commerical, applies to this acquisition except that there is no SF 1449 solicitation cover sheet. A blank form of the representations and certifications document, which must be completed as required by 52-212-4, Contract terms and Conditions-Commerical Items applies to this acquisition. The clause at 52-212-5, Contract Terms and Conditions Required to Implement Statues or Executive Orders-Commerical Items applies to this acquisition. The clauses of subpart (b), which apply, are 6 and 10. Any response is due no later than 3:00 p.m., Eastern Standard Time, September 9, 1998, to Linda Taylor. For delivery through the postal service the address is room 1 C 38, Solar Building, NIH/NIAID, 6003 Executive Blvd., Rockville, Md 20852. Electronic submission will not be accepted. Request for information concerning this requirement is to be address to Linda Taylor, 301-402-2282. Collect calls will not be accepted. Posted 08/07/98 (W-SN234202). (0219)

Loren Data Corp. http://www.ld.com (SYN# 0369 19980811\66-0017.SOL)