National Cancer Institute, Research Contracts Branch, PSAS, 6120 Executive Blvd, EPS/Room 638, Bethesda, MD 20892-7227

66 -- SKY VISON/H CYTOGENETICS WORKSTATION SOL RFQ80202(NR) DUE 090398 POC Marsha Gorham, Purchasing Agent (301)402-4509 or Todd Cole, Contracting Officer The National Cancer Institute (NCI) plans to procure a SKY Vision/H Cytogenetics Workstation with appropriate spectrometer, software package, light source and required filter sets, from Applied Spectral Imaging Inc., 2120 Las Palmas Dr., Carlsberg, CA 92009. The system shall be assembled on an existing ZEISS research epifluorescent microscope, and it will be used to access balanced chromosomal rearrangements in human and mouse karyotypes on a global basis through spectral karyotyping and thus identify recurrent chromosomal breakpoints. The system shall: 1) be capable of automatic karyotyping of human (24 colors) and mouse (21 colors) metaphase chromosome spreads, based upon a single hybridization with appropriate combinatorial -labeled DNA probes, and one-pass spectral readout from the specimen; 2) employ algorithms using the fluorescence spectra of the pure dyes used in DNA-probe labeling thus avoiding any prior identification of chromosomes; 3) be based upon an imaging, fourier-transform spectrometer attachable via C-mount to the Laboratory's existing Zeiss microscope; 4) have an optical head that shall employ a common-path interferometer that ensures the system is stable, durable and easily calibrated with any standard source or set of narrow band interference filters; 5) have optical path difference (OPD) for the Optical Head that is controlled by a variable speed, high accuracy, digital position-controlled qalvanometer; 6) to enable 24/21 color karyotyping, have broad spectra response from 400 nm to 1000 nm, high variable spectra resolution adjustable by the user from inside the software and spatial resolution limited only by pixel size; 7) have an interferometer that does not degrade achievable and spatial resolutions; 8) include thermoelectrically cooled, low noise, high dynamic range, progressive scan interline CCD camera with 659 X 494 pixels, stand alone camera controller and rasterizing (frame grabber) computer board; 9) provide images that are acquired automatically in the interferometer, fourier-transformed in no longer than 2 minutes and rapidly transferred at a rate of 1 M pixel/sec or better in digital format to the RAM of the computer via direct memory access. The system software shall enable several types of interferometer/CCD calibrations, including fringe and spectral calibration, as well as optical alignment. It should provide the ability to define multiple reference spectra for storage in a reference library and/or for incorporation in spectrally based (minimum square error) classification algorithms and (spectral) optical density algorithms. Spectral algorithms should be applicable to images based on a number of spectra to be chosen from a reference library and/or from within an image of interest. It also should have the ability to do spectral browsing' to view and display in real time spectra measured at any pixel in the image being viewed. The Software shall also display images in CIE corrected RGB colors, effectively combine the G- or DAPI-banded image with multi-color spectral and keryotyping information and human or mouse chromosome ideograms, save images in more than one file format, export output spectral data to ASCII files, and do wavelength scanning, as well as spectral & spatial averaging. In addition, the user shall be able to perform basic arithmetic image transformations, to add, subtract, divide and multiply spectral images and to view the results immediately. Applied Spectral Imaging Inc., is the only source known to NCI that offers an interferometer-based system that uses one-pass accurate spectral readout to automatically reconstruct 24 color human and 21 color mouse karyotypes. However, if any interest party believes it can meet the above requirement, it may submit a statement of capabilities. The capability statement and any other information furnished must be in writing and must contain material in sufficient detail to allow NCI to determine if the party can meet the requirement. Capability statements must be received in the contracting office by 3:00 p.m. EST, onSeptember 3, 1998. If you have any questions, please contact Marsha Gorham, Purchasing Agent, on (301)402-4509. A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. No collect calls will be accepted. Posted 08/18/98 (W-SN238357). (0230)

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