66 -- INSTRUMENTS & LABORATORY EQUIPMENT

Notice Date

April 27, 2001

Contracting Office

National Institute of Environmental Health Sciences, Acquisitions Management Branch, 79 T.W. Alexander Drive, Building 4401 Research Commons, P.O. Box 12874, Research Triangle Park, NC 27709

ZIP Code

27709

Solicitation Number

273-01-P-0007

Response Due

June 16, 2001

Point of Contact

Contact Pamela Evans, Contract Specialist, (919) 541-0401

Description

The National Institute of Environmental Health Sciences (NIEHS) intends to purchase One Hybrid Quadrupole-Time-of-Flight Tandem Mass Spectrometer with both a Matrix-Assisted Laser Desorption/Ionization Source (MALDI-TOF) and a (nano) Electrospray Source (ESI) for the Identification of Protein Digests. Specific potential applications of the instrument include, but are not limited to: identification of posttranslationally modified peptides, especially phosphopeptides, from tryptic digests; identification of the sites of modification of posttranslationally modified peptides; MS/MS based identification of proteolytic digests of proteins and protein mixtures; The instrument must have a hybrid quadrupole/time-of-flight analyzer. Mass Range of 2-10000 is required. Primary ionization source-matrix-assisted laser desorption/ionization capable of accommodating PEBiosystems MALDI targets. Secondary ionization source-nano electrospray source. Routine negative ion capabilities under data systems control. Mass accuracy >20 ppm with an external calibration; Mass resolution of MS/MS scans -- 10,000; Sensitivity-Interpretable MS/MS spectra should be obtainable from 200 femtomole peptide sample. MS/MS modes-Interpretable daughter ion; Software should be capable of detecting an LC peak, selecting a molecular ion and initiating an MS/MS scan for HPLC separations combined with ESI. Integrated biological application software package is recommended. Software site-license, System installation, startup and initial on-site instructions shall be included. One-year warrant from date of acceptance of complete order is required. Foreground/background data system operation is recommended. Used equipment is not acceptable. This is a synopsis/solicitation for commercial items in accordance with FAR 12.6. Sources may submit descriptive literature which fully demonstrate that their product meets the required specifications. Issue Date: 5/16/01

Record

Loren Data Corp. 20010501/66SOL012.HTM (W-117 SN50K5L6)