68 -- MEASUREMENT OF HUMAN EXPOSURE TO VARIOUS NITROGEN MUSTARD

Notice Date

May 2, 2001

Contracting Office

Department of Health and Human Services, Center for Disease Control and Prevention, Procurement and Grants Office (Atlanta), 2920 Brandywine Road, Room 3000, Atlanta, GA, 30341-4146

ZIP Code

30341-4146

Solicitation Number

Reference-Number-AT15501

Response Due

May 17, 2001

Point of Contact

Berta Hollenberger, Contract Specialist, Phone 770-488-2643, Fax 770-488-2670, Email boh9@cdc.gov

E-Mail Address

Berta Hollenberger (boh9@cdc.gov)

Description

THIS IS A COMBINED SYNOPSIS/SOLICITATION FOR COMMERCIAL ITEMS PREPARED IN ACCORDANCE WITH THE FORMAT IN SUBPART 12.6, AS SUPPLEMENTED WITH ADDITIONAL INFORMATION INCLUDED IN THIS NOTICE. THIS ANNOUNCEMENT CONSTITUTES THE ONLY SOLICITATION; QUOTES ARE BEING REQUESTED AND A WRITTEN SOLICITATION WILL NOT BE ISSUED. AT15501 is being issued as a request for quotes (RFQ). RFQ AT15501 and incorporated provisions and clauses are those in effect through Federal Acquisition Circular (FAC)97-23. The associated NAICS code is 541710 and the small business size standard is 500 employees. The Centers for Disease Control & Prevention/National Center for Environmental Health, requires tripeptide nitrogen mustard adducts and albumin samples from human blood exposed to HN1 Bis(2-chloro-ethyl)ethyl amine CAS #538-07-8, HN2 Bis(2-chloro-ethyl)methyl amine CAS #51-75-2, and HN3 Tris(2-chloro-ethyl)amine CAS#555-77-1 and Propyl-nitrogen mustard (internal standard). The following tripeptide nitrogen mustard adducts will be derived from human serum albumin and delivered as neat compounds in screw?cap vials: 1) Cys(HN1)-Pro-Phe, 1 mg; 2) Cys(HN2)-Pro-Phe, 1mg; 3) Cys(HN3)-Pro-Phe, 1 mg; 4) Cys(Propyl-nitrogen mustard)-Pro-Phe, 1 mg. The results of mass spectral analysis will be provided for the above compounds. Albumin samples will be obtained from human blood after exposure to HN1, HN2, HN3 and Propyl-nitrogen mustard at the following concentrations: 0.1 mM HN1; 1 mM HN1; 0.1 mM HN2; 1 mM HN2; 0.1 mM HN3; 1 mM HN3; 0.1 mM Propyl-nitrogen mustard; 1 mM Propyl-nitrogen mustard. Exposed albumin samples shall be delivered as solids in 25 mg quantities in screw-cap vials. Mass spectral analysis shall be performed after Pronase digestion to verify the presence of the tripeptide adducts and the results will be provided. To the best of our knowledge, the tripeptide?nitrogen mustard adducts and albumin-nitrogen mustard adducts are specialty compounds not in general use. Additionally, the starting materials HN1, HN2, and HN3 are very reactive and extremely toxic. Only institutions involved in specific research projects associated with nitrogen mustards have the necessary training for the safe handling of these compounds. The method for measuring nitrogen mustard exposure in humans was developed at the Netherlands Organization for Applied Scientific Research TNO facility. Accordingly, the standards and quality control materials necessary to validate this method were also developed at this facility and these compounds are part of their inventory. Therefore, CDC believes that The Netherlands Organization for Applied Scientific Research TNO located in Lange Kleiweg 137, P.O. Box 45, 2280 AA Rijswijk, The Netherlands, is the only institution in possession of these materials. As a result, CDC intends to issue a sole source contract with this company. However, all responsible sources that can satisfy the above requirements may submit a quote, which will be considered by the agency. Quoters shall submit prices for one each of the following 12 items: 1) Cys(HN1)-Pro-Phe is a Bis(2-chloroethyl)ethylamine adduct of the tripeptide Cysteine-Proline-Phenanaline, formula: NH2CH[CH2SCH2CH2N(CH2CH3)CH2CH2OH]C(O)-Proline-Phenanaline(COOH); 2) Cys(HN2)-Pro-Phe is a Bis(2-chloroethyl)methylamine adduct of the tripeptide Cysteine-Proline-Phenanaline, formula: NH2CH[CH2SCH2CH2N(CH3)CH2CH2OH]C(O)-Proline-Phenanaline(COOH); 3) Cys(HN3)-Pro-Phe is a Tris(2-chloroethyl)amine adduct of the tripeptide Cysteine-Proline-Phenanaline, formula: NH2CH[CH2SCH2CH2N(CH2CH2OH)CH2CH2OH]C(O)-Proline-Phenanaline(COOH); 4) Cys(Propyl-nitrogen mustard)-Pro-Phe is a Bis(2-chloroethyl)proplyamine adduct of the tripeptide Cysteine-Proline-Phenanaline, formula: NH2CH[CH2SCH2CH2N(CH2CH2CH3)CH2CH2OH]C(O)-Proline-Phenanaline(COOH); 5) Human albumin-HN1 adduct at 0.1mM level is a Bis(2-chloroethyl)ethylamine adduct of human albumin at an exposure of of 0.1 mM of Bis(2-chloroethyl)ethylamine; 6) Human albumin-HN1 adduct at 1:M level is a Bis(2-chloroethyl)ethylamine adduct of human albumin at an exposure of of 1 :M of Bis(2-chloroethyl)ethylamine; 7) Human albumin-HN2 adduct at 0.1mM level is a Bis(2-chloroethyl)methylamine adduct of human albumin at an exposure of of 0.1 mM of Bis(2-chloroethyl)methylamine; 8) Human albumin-HN2 adduct at 1:M level is a Bis(2-chloroethyl)methylamine adduct of human albumin at an exposure of of 1 :M of Bis(2-chloroethyl)methylamine; 9) Human albumin-HN3 adduct at 0.1mM level is a Tris(2-chloroethyl)amine adduct of human albumin at an exposure of of 0.1 mM of Tris(2-chloroethyl)amine; 10) Human albumin-HN3 adduct at 1:M level is a Tris(2-chloroethyl)amine adduct of human albumin at an exposure of of 1 :M of Tris(2-chloroethyl)amine; 11) Human albumin-propyl-nitrogen mustard adduct at 0.1mM level is a Bis(2-chloroethyl)propylamine adduct of human albumin at an exposure of of 0.1 mM of Bis(2-chloroethyl)propylamine; 12) Human albumin-propyl-nitrogen mustard adduct at 1:M level is a Bis(2-chloroethyl)propylamine adduct of human albumin at an exposure of of 1 :M of Bis(2-chloroethyl)propylamine. Quoters shall also submit, as part of their quotes, the following information for evaluation (see evaluation criteria below): (a)Mass spectra of required nitrogen mustard-tripeptide adducts; and (b) list of projects involving the nitrogen mustards HN1, HN2 and HN3. Include in list of projects, name of company and complete address, name of contact, telephone and fax numbers, and e-mail address. CDC may contact some or all of the contacts listed to verify quoters' past performance. Quoters are responsible for providing accurate and complete information. (Failure to do so may cause rejection of the quote.) Delivery of all items shall be made within six (6) months after receipt of order, FOB Destination, Within Consignee's Premises to: CDC-NCEH-CENTRAL RCG, Bldg. 17 Loading Dock-4770 Buford Hwy. NE, Atlanta, GA 30341. FAR provisions 52.212-1, Instruction to OfferorsBCommercial Items, and 52.212-3, Offeror Representations and CertificationsBCommercial Items, apply to this RFQ. The following FAR clauses apply: 52.212-2, EvaluationBCommercial items (with the following evaluation criteria: (a) Demonstrated experience in the handling of the nitrogen mustards HN1, HN2, and HN3 and the synthesis of these nitrogen mustard-albumin adducts; (b) Ability to provide mass spectral analysis to verify structure of compounds and presence of tripeptide adducts; (c) Past performance; and (d)Price. (The non-price factors above when combined are significantly more important than price.) FAR 52.212-4, Contract Terms and ConditionsBCommercial Items (with addendum incorporating FAR 52.232-35, Designation of Office for Government Receipt of Electronic Funds Transfer Information (The designated office is: CDC, Attn.: Financial Management Office; P.O. Box 15580; Atlanta, GA 30333; Tel.: (404)687-6666); and FAR 52.247-35, F.O.B. Destination, Within Consignee's Premises. FAR 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive OrdersBCommercial Items, with the following cited FAR clauses applicable: FAR 52.203-6,Restrictions on Subcontractor Sales to the Government, with Alt. I; FAR 52.219-8, Utilization of Small Business Concerns; FAR 222-21, Prohibition of Segregated Facilities; FAR 52.222-26, Equal Opportunity; FAR 52-222-35, Affirmative Action for Disabled Veterans and Veterans of the Vietnam Era; FAR 52.222-36, Affirmative Action for Workers with Disabilities; FAR 52.222-37, Employment Reports on Special Disabled Veterans and Veterans of the Vietnam Era; FAR 52.222-19, Child LaborCCooperation with Authorities and Remedies; FAR 52.225-5, Trade Agreements Act; FAR 52.225-13, Restriction on Certain Foreign Purchases and, FAR 52.232-34, Payment by Funds Transfer -- -Other than Central Contractor Registration. In addition to price and the information required for evaluation above, quoters shall submit a copy of FAR provision 52.212-3 fully executed. All FAR clauses and provisions can be obtained at: www.arnet.gov/far/facsframe.html. The Defense Priorities and Allocations System (DPAS) does not apply. Quotes are due at 2:00 PM (local time), May 17, 2001 to the following address: Centers for Disease Control & Prevention, Procurement & Grants Office, Contracts Management Branch, 2920 Brandywine Road, Atlanta, GA 30341-4164. Facsimile quotes are not authorized. Technical inquiries shall be e-mailed to boh9@cdc.gov. Quotes received after the time and date set for receipt of quotes will not be considered for award.*****

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Loren Data Corp. 20010504/68SOL006.HTM (D-122 SN50K947)